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Restriction Endonucleases > Hph I

Hph I


 
 
 Recognition site:
 
 GGTGA(N)8
CCACT(N)7
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
100% 0-20% 0-20% 0-20% 20-50% 0-20%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
NR 75% 0% 100%
 
 Recommended buffer: 
Fermentas:Buffer B+
NEB:NEBuffer 4
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Hph I, Fermentas
Hph I, NEB
 
 


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