Science Gateway

Protocols: ES Cell Culture - Gene Targeting - Protocols

source: Bradley Laboratory Homepage

Tissue Culture Protocols

  • General ES Cell Protocols/Starting Out With ES Cells


  • Recipes for Tissue Culture Selective Drug Stocks


  • Components and Supplementation of Various Culture Media


  • Electroporation into ES cells


  • Analysis of ES Cell Clones by Mini-Southern


  • Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes


  • Picking ES Cell Colonies and Transferring Them to 96-Well PLATES


  • Use of the Hemacytometer for the Determination of Cell Numbers


  • beta-Galactosidase Color Assay for Cultured Cells


  • Isolation of Primary Fibroblasts from Mouse Embryos


  • Preparation of Feeder Layers And SNL Stocks


  • Preparing 48-Well Plate Feeders


  • Frozen Feeders


  • Freezing and Thawing Cultured Cells


  • Feeder Preparation by Gamma Irration


  • Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates


  • Preparation of RNA from Cultured Cells


    Other Protocols

  • Colony Hybridization


  • Genomic DNA:Restriction Enzyme Digests, Agarose Gel Electrophoresis, and Southern Transfer (Blotting)


  • Transfer of DNA to GeneScreen Plus Using the Bio-Dot SF Apparatus