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PROW: CDw108
[PROW BAR]
PROW and IWHLDA present the GUIDE on:
CDw108
Author: Marilyn Telen
Reviewer: Vaclav Horejsi
Link to additional info in FORUM
[GUIDE BAR]

OVERVIEW
MOLECULAR FAMILIES  

ALTERNATE NAMES  

  • SEMA7A [HUGO gene name]
  • John-Milton-Hagen, JMH human blood group antigen

    MAJOR LINKS  

  • NCBI LocusLink Record: 8482
  • AUTHOR'S ADDITIONAL INSIGHTS  

  • Autoantibodies to this protein are relatively common and often associated with loss of JMH antigen expression by circulating red cells
  • CDw108 can also be released from cells as a 67 kDa protein through proteolysis, however, soluble CDw108 is not normally present in plasma
    SYSTEM VIEW
    DISEASE RELEVANCE / FUNCTION IN INTACT  
  • In a preliminary report, deficiency of erythroid CDw108 has been reported as associated with a rare form of congenital dyserythropoietic anemia

    CELLULAR FUNCTION  

  • An adhesion function has been postulated but not proven for CDw108

    CELLULAR EXPRESSION  

  • Expressed on erythrocytes
  • Expressed at low levels on circulating lymphocytes
  • Expressed at moderately high levels by lymphoblasts and lymphoblastic cell lines
    GENE
    TRANSCRIPTION REGULATING MOLECULES  
    - Not known


    TRANSCRIPT
    POST-TRANSCRIPTIONAL MODIFICATION  
    - Not known


    PROTEIN
    BIOCHEMICAL ACTIVITY  
    - Not known

    PROTEIN SEQUENCE ANNOTATION Link to additional info in FORUM  

  • CDw108 is a glycosylphosphatidylinositol (GPI)-linked protein whose primary structure remains unknown

    PROTEIN PHYSICAL PROPERTIES  

    CELL TYPEMW UNREDUCEDMW REDUCEDComment
    Lymphocytes 76 kDa 80 kDa  
    Erythrocytes 76 kDa 80 kDa  

    POST-TRANSLATIONAL MODIFICATION  

  • Approximately 20% of the apparent molecular weight is accounted for by N-linked carbohydrate, as determined by endoglycosidase F treatment
    MOLECULAR INTERACTIONS
    SUBSTRATES  
    - Not known

    ENZYME FOR WHICH THIS IS A SUBSTRATE  
    - Not known

    OTHER LIGANDS AND ASSOCIATED MOLECULES Link to additional info in FORUM  

    MOLECULECOMMENT
    Tyrosine kinases Antibodies to CDw108 coprecipitate protein kinases, most likely tyrosine kinases belonging to the src family

    REAGENTS
    MABS NEW  
    - No information

    MABS REFERENCE  

    NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
    NL-156 V Horejsi, Czech Republic  
    MEM-121   {Klickstein_LB/Springer_TA..LTV}
    MEM-150   {Klickstein_LB/Springer_TA..LTV}
    H8   {Daniels_GL/Knowles_RW.1982.JI}, {Daniels_GL/Knowles_RW.1983.JI}
  • After the Workshop conference it was found in the reviewer's laboratory that workshop samples N-L153 (H105) and N-L154 (KS.2) (both submitted by K.Sagawa) completely cross-block CDw108 mAb MEM-150. There is a paper (with English summary) on the H105 mAb in a Japanese journal {Kubo_K.1991.KMJ}; the properties of the antigen are compatible with CDw108
    REFERENCES
    REVIEWS  
    1. {Klickstein_LB/Springer_TA..LTV}
    Klickstein LB and Springer TA. . CDw108 cluster report. In Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, Ritz J, Shaw S, Silverstein R, Springer T, Tedder TF and Todd RF. 1995. Leukocyte Typing V. OXFORD University Press p1477

    PRIMARY CITATIONS  
    2. {Bobolis_KA/Telen_MJ.1991.TRANS}
    Bobolis KA and Telen MJ. 1991. Bobolis KA, Telen MJ. 1991. Transfusion 31:46S. Transfusion 31:46S
    3. {Kubo_K.1991.KMJ}
    Kubo K. 1991. Kubo K. 1991. J. Kurume Med. Assoc. 12: 1051-1060. Kurume Med J 12:1051**
    4. {Bobolis_KA/Telen_MJ.1992.BLOOD}
    Bobolis KA, Moulds JJ and Telen MJ. 1992. Isolation of the JMH antigen on a novel phosphatidylinositol-linked human membrane protein. Blood 79:1574-1581
    5. {Daniels_GL/Knowles_RW.1982.JI}
    Daniels GL and Knowles RW. 1982. A monoclonal antibody to the high frequency red cell antigen JMH. J Immunogenet 9:57-59
    6. {Daniels_GL/Knowles_RW.1983.JI}
    Daniels GL and Knowles RW. 1983. Further analysis of the monoclonal antibody H8 demonstrating a JMH-related specificity. J Immunogenet 10:257-258
    7. {Mudad_R/Telen_MJ.1995.TP}
    Mudad R, Rao N, Angelisova P, Horejsi V and Telen MJ. 1995. Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion (Paris) 35:566-570
    8. {Mudad_R/Telen_MJ.1995.TP}
    Mudad R, Rao N, Issitt PD, Roy RB, Combs MR and Telen MJ. 1995. JMH variants: serologic, clinical, and biochemical analyses in two cases. Transfusion (Paris) 35:925-930

    WWW RESOURCES  


    * indicates amended by reviewer, ** indicates added by reviewer
    Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission
    Modified 01/25/00   mpr@mail.nih.gov