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PROW: CD35
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PROW and IWHLDA present the GUIDE on:
CD35
Authors: Michael Nickells; John P. Atkinson
Reviewer: Gordon Ross
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ALTERNATE NAMES FOR CD35  

MAJOR LINKS FOR CD35  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD35  

CELLULAR FUNCTION OF CD35  

DISEASE RELEVANCE OF CD35 AND FUNCTION OF CD35 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD35  

MOLECULAR STRUCTURE OF CD35  

MOLECULAR MASS OF CD35  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
Erythrocyte 160, 190, 220, 250 kDa 190, 220, 250, 280 kDa Molecular mass polymorphism due to codominant expression of alleles with gene frequencies of: 0.01, 190 kDa; 0.83, 220 kDa; 0.16, 250 kDa; and <0.01, 280 kDa (reduced)
Leukocytes 165, 195, 225, 255 kDa 195, 225, 255, 285 kDa There is approximately 5 kDa increase in molecular mass on leukocytes due to glycosylation

POST-TRANSCRIPTIONAL MODIFICATION OF CD35  

POST-TRANSLATIONAL MODIFICATION OF CD35  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD35   - No information

SUBSTRATES FOR CD35  

ENZYMES WHICH MODIFY CD35   - No information

LIGANDS FOR CD35 AND MOLECULES ASSOCIATED WITH CD35  

MOLECULECOMMENT
C3b C3b is a primary natural ligand. CD35's affinity is greater for C3b than for C4b, weaker affinity is seen with iC3b and C3dg. In vitro, affinities are enhanced by reducing the ionic strength
C4b C4b is a primary natural ligand. CD35's affinity is greater for C3b than for C4b, weaker affinity is seen with iC3b and C3dg. In vitro, affinities are enhanced by reducing the ionic strength
iC3b CD35's affinity is greater for C3b than for C4b, weaker affinity is seen with iC3b and C3dg. In vitro, affinities are enhanced by reducing the ionic strength
C3dg CD35's affinity is greater for C3b than for C4b, weaker affinity is seen with iC3b and C3dg. In vitro, affinities are enhanced by reducing the ionic strength
iC3 (hemolytically inactive C3, C3(H20), C3u) Binds CD35 with affinity similar to C3b and C4b. In vitro, affinities are enhanced by reducing the ionic strength
iC4 (hemolytically inactive C4, C4 (H2O), C4u) Binds CD35 with affinity similar to C3b and C4b. In vitro, affinities are enhanced by reducing the ionic strength

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD35  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD35   - No information


REAGENTS
CD35-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP   - No information

SELECTION OF OTHER CD35-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
7G9 Reist et al. 1994  
HB8592 Tausk et al. 1986  
YZ-1 Changelian et al. 1985  
1B4O Shea et al. 1985  
KuN241 Mathew et al. 1995  
9H3 RP Taylor, University of Virginia School of Medicine, Charlottesville, VA  
4D6.1 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
4D12.1 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
8C9.1 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
9A3.1 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
1F11.G12 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
3C6.D11 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  
6B1.H12 HC Marsh, Jr., T Cell Sciences, Inc., Needham, MA  

 
SELECTED REFERENCES ON CD35  

REVIEWS

1. Ahearn JM,Fearon DT Structure and function of the complement receptors, CR1 (CD35) and CR2 (CD21). Adv Immunol 1989 46:183 PubMed

2. Hourcade D,Holers VM,Atkinson JP The regulators of complement activation (RCA) gene cluster. Adv Immunol 1989 45:381 PubMed

3. Moore FD Jr Therapeutic regulation of the complement system in acute injury states. Adv Immunol 1994 56:267 PubMed

4. Ross GD,Medof ME Membrane complement receptors specific for bound fragments of C3. Adv Immunol 1985 37:217 PubMed

5. Taylor RP,Ferguson PJ Primate erythrocyte (E) complement receptor (CR1) as an anchor site for bispecific-based therapies to clear pathogens or autoantibodies safely from the circulation. J Hematother 1995 4:357 PubMed

PRIMARY CITATIONS

6. Birmingham DJ,Logar CM,Shen XP,Chen W The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element. J Immunol 1996 157:2586 PubMed

7. Changelian PS,Jack RM,Collins LA,Fearon DT PMA induces the ligand-independent internalization of CR1 on human neutrophils. J Immunol 1985 134:1851 PubMed

8. Kalli KR,Hsu PH,Bartow TJ,Ahearn JM,Matsumoto AK,Klickstein LB,Fearon DT Mapping of the C3b-binding site of CR1 and construction of a (CR1)2- F(ab')2 chimeric complement inhibitor. J Exp Med 1991 174:1451 PubMed

9. Krych M,Clemenza L,Howdeshell D,Hauhart R,Hourcade D,Atkinson JP Analysis of the functional domains of complement receptor type 1 (C3b/C4b receptor; CD35) by substitution mutagenesis. J Biol Chem 1994 269:13273 PubMed

10. Mathew JM,Naziruddin B,Duffy B,Krych M,Mohanakumar T Functional analysis of complement receptor 1 using a new monoclonal antibody, KuN241. Hybridoma 1995 14:29 PubMed

11. Molina H,Holers VM,Li B,Fung Y,Mariathasan S,Goellner J,Strauss-Schoenberger J,Karr RW,Chaplin DD Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2. Proc Natl Acad Sci U S A 1996 93:3357 PubMed

12. Moulds JM,Nickells MW,Moulds JJ,Brown MC,Atkinson JP The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. J Exp Med 1991 173:1159 PubMed

13. Nickells MW,Subramanian VB,Clemenza L,Atkinson JP Identification of complement receptor type 1-related proteins on primate erythrocytes. J Immunol 1995 154:2829 PubMed

14. O'Shea JJ,Brown EJ,Seligmann BE,Metcalf JA,Frank MM,Gallin JI Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. J Immunol 1985 134:2580 PubMed

15. Reist CJ,Liang HY,Denny D,Martin EN,Scheld WM,Taylor RP Cross-linked bispecific monoclonal antibody heteropolymers facilitate the clearance of human IgM from the circulation of squirrel monkeys. Eur J Immunol 1994 24:2018 PubMed

16. Tausk FA,McCutchan A,Spechko P,Schreiber RD,Gigli I Altered erythrocyte C3b receptor expression, immune complexes, and complement activation in homosexual men in varying risk groups for acquired immune deficiency syndrome. J Clin Invest 1986 78:977 PubMed

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Modified 10/14/99   mpr@mail.nih.gov