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PROW: CD135
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PROW and IWHLDA present the GUIDE on:
CD135
Author: Hans-Joerg Buehring
Reviewer: Stewart Lyman
[GUIDE BAR]

ALTERNATE NAMES FOR CD135  

MAJOR LINKS FOR CD135  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD135  

CELLULAR FUNCTION OF CD135  

DISEASE RELEVANCE OF CD135 AND FUNCTION OF CD135 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD135  

MOLECULAR STRUCTURE OF CD135  

MOLECULAR MASS OF CD135  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
Ba/F3 cells transfected with the human FLT3 cDNA 130 kDa 155 kDa  
Leukemic cells 130 kDa 155/160 kDa  

POST-TRANSCRIPTIONAL MODIFICATION OF CD135  

POST-TRANSLATIONAL MODIFICATION OF CD135  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD135   - Not known

SUBSTRATES FOR CD135  

MOLECULECOMMENT
GAP Associates with CD135, activated by phosphorylation
PLCg Associates with CD135, activated by phosphorylation
VAV Seems not to associate with CD135 but is phosphorylated after CD135 activation
SHC Seems not to associate with CD135 but is phosphorylated after CD135 activation
PI3-kinase Associates through SH2 domains to Tyr-phosphorylated site on the carboxy tail of CD135
GRB2 Associates through SH2 domains to Tyr-phosphorylated site on the carboxy tail of CD135

ENZYMES WHICH MODIFY CD135  

LIGANDS FOR CD135 AND MOLECULES ASSOCIATED WITH CD135  

MOLECULECOMMENT
FL (FLT3 ligand) Type I transmembrane protein with an N-terminal signal peptide of 26aa, followed by a 156-aa extracellular region, a 23-aa transmembrane domain, and a short 30-aa cytoplasmic region. Alternative mRNA splicing produces membrane-bound and soluble isoforms (both active). Synergizes with other cytokines for proliferation and differentiation of hematopoietic progenitor cells

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD135  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD135  
REAGENTS
CD135-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
4G8 HJ Bühring, University of Tübingen, Germany  
BV10 HJ Bühring  
SF1.340 O Rosnet, U.119 INSERM, Marseille, France  
SF1.394 O Rosnet  

SELECTION OF OTHER CD135-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
M22 SD Lyman, Immunex Corporation, Seattle, WA  
M25 SD Lyman  
M27 SD Lyman  
M29 SD Lyman  

 
SELECTED REFERENCES ON CD135  

REVIEWS

1. Drexler HG Expression of FLT3 receptor and response to FLT3 ligand by leukemic cells. Leukemia 1996 10:588 PubMed

2. Lyman, SD et al. 1995 Curr Opin Hematol 2: 177-181

3. Rosnet O,Buhring HJ,deLapeyriere O,Beslu N,Lavagna C,Marchetto S,Rappold I,Drexler HG,Birg F,Rottapel R,Hannum C,Dubreuil P,Birnbaum D Expression and signal transduction of the FLT3 tyrosine kinase receptor. Acta Haematol 1996 95:218 PubMed

PRIMARY CITATIONS

4. Lavagna C,Marchetto S,Birnbaum D,Rosnet O Identification and characterization of a functional murine FLT3 isoform produced by exon skipping. J Biol Chem 1995 270:3165 PubMed

5. Lyman SD,James L,Johnson L,Brasel K,de Vries P,Escobar SS,Downey H,Splett RR,Beckmann MP,McKenna HJ Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells. Blood 1994 83:2795 PubMed

6. Mackarehtschian K,Hardin JD,Moore KA,Boast S,Goff SP,Lemischka IR Targeted disruption of the flk2/flt3 gene leads to deficiencies in primitive hematopoietic progenitors. Immunity 1995 3:147 PubMed

7. Matthews W,Jordan CT,Wiegand GW,Pardoll D,Lemischka IR A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 1991 65:1143 PubMed

8. Rappold I,Ziegler BL,Kohler I,Marchetto S,Rosnet O,Birnbaum D,Simmons PJ,Zannettino AC,Hill B,Neu S,Knapp W,Alitalo R,Alitalo K,Ullrich A,Kanz L,Buhring HJ Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood 1997 90:111 PubMed

9. Rosnet O,Buhring HJ,Marchetto S,Rappold I,Lavagna C,Sainty D,Arnoulet C,Chabannon C,Kanz L,Hannum C,Birnbaum D Human FLT3/FLK2 receptor tyrosine kinase is expressed at the surface of normal and malignant hematopoietic cells. Leukemia 1996 10:238 PubMed

10. Rosnet O,Marchetto S,deLapeyriere O,Birnbaum D Murine Flt3, a gene encoding a novel tyrosine kinase receptor of the PDGFR/CSF1R family. Oncogene 1991 6:1641 PubMed

11. Rosnet O,Schiff C,Pebusque MJ,Marchetto S,Tonnelle C,Toiron Y,Birg F,Birnbaum D Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993 82:1110 PubMed

12. Rusten LS,Lyman SD,Veiby OP,Jacobsen SE The FLT3 ligand is a direct and potent stimulator of the growth of primitive and committed human CD34+ bone marrow progenitor cells in vitro. Blood 1996 87:1317 PubMed

13. Shah AJ,Smogorzewska EM,Hannum C,Crooks GM Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro. Blood 1996 87:3563 PubMed

14. Small D,Levenstein M,Kim E,Carow C,Amin S,Rockwell P,Witte L,Burrow C,Ratajczak MZ,Gewirtz AM,et al STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34+ human bone marrow cells and is involved in the proliferation of early progenitor/stem cells. Proc Natl Acad Sci U S A 1994 91:459 PubMed

15. Zeigler FC,Bennett BD,Jordan CT,Spencer SD,Baumhueter S,Carroll KJ,Hooley J,Bauer K,Matthews W Cellular and molecular characterization of the role of the flk-2/flt-3 receptor tyrosine kinase in hematopoietic stem cells. Blood 1994 84:2422 PubMed

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Modified 10/14/99   mpr@mail.nih.gov