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PROW: CD9
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PROW and IWHLDA present the GUIDE on:
CD9
Authors: Eric Rubinstein; Masja de Haas
Reviewer: Andrew R. Shaw
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ALTERNATE NAMES FOR CD9  

MAJOR LINKS FOR CD9  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD9  

CELLULAR FUNCTION OF CD9  

DISEASE RELEVANCE OF CD9 AND FUNCTION OF CD9 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD9  

MOLECULAR STRUCTURE OF CD9  

MOLECULAR MASS OF CD9  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
All   24 kDa (major band), 26 kDa (minor band) Differentiating B cells (Yashukawa et al. 1988;Zola et al. 1991, 1989)**

POST-TRANSCRIPTIONAL MODIFICATION OF CD9   - No information

POST-TRANSLATIONAL MODIFICATION OF CD9  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD9  
MOLECULECOMMENT
SP1 SP1 has been shown to bind to the CD9 promoter

SUBSTRATES FOR CD9   - No information

ENZYMES WHICH MODIFY CD9   - No information

LIGANDS FOR CD9 AND MOLECULES ASSOCIATED WITH CD9  

MOLECULECOMMENT
CD63 CD9 associates with other members of the tetraspan superfamily (CD63, CD81, CD82)
CD81 CD9 associates with other members of the tetraspan superfamily (CD63, CD81, CD82)
CD82 CD9 associates with other members of the tetraspan superfamily (CD63, CD81, CD82)
Beta-1 integrins In various cell line
CD41/CD61 (gpIIb/IIIa) Associates with CD9 in platelets (Slupsky et al. 1989)**
HLA-DR In pre-B cells and transfected B cell lines
HB-EGF (heparin-binding EGF) CD9 is also associated with the transmembrane precursor form of a growth factor, HB-EGF (heparin-binding EGF)
PI4 kinase CD9 is associated with PI4 kinase (Martin Hemler-personal communication)**

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD9  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD9  
REAGENTS
CD9-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
MEM-192 V Horejsi, Prague  
MEM-61 V Horejsi, Prague  
SN4 BK Seon, Buffalo  
SN4d B K Seon, Buffalo  

SELECTION OF OTHER CD9-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
ALB-6 Boucheix, Villejuif, France  
BA-2 Kersey and LeBien, Minneapolis, MN  
50H19 Shaw, Edmonton, Canada  
mab7 Jennings, Memphis TN  

 
SELECTED REFERENCES ON CD9  

REVIEWS

1. Rubinstein E,Boucheix C,Worthington RE,Carroll RC Anti-platelet antibody interactions with Fc gamma receptor. Semin Thromb Hemost 1995 21:10 PubMed

2. Wright MD,Tomlinson MG The ins and outs of the transmembrane 4 superfamily. Immunol Today 1994 15:588 PubMed

PRIMARY CITATIONS

3. Berditchevski F,Zutter MM,Hemler ME Characterization of novel complexes on the cell surface between integrins and proteins with 4 transmembrane domains (TM4 proteins). Mol Biol Cell 1996 7:193 PubMed

4. Boucheix C,Benoit P,Frachet P,Billard M,Worthington RE,Gagnon J,Uzan G Molecular cloning of the CD9 antigen. A new family of cell surface proteins. J Biol Chem 1991 266:117 PubMed

5. Ikeyama S,Koyama M,Yamaoko M,Sasada R,Miyake M Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA. J Exp Med 1993 177:1231 PubMed

6. Iwamoto R,Higashiyama S,Mitamura T,Taniguchi N,Klagsbrun M,Mekada E Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin sensitivity. EMBO J 1994 13:2322 PubMed

7. Lanza F,Wolf D,Fox CF,Kieffer N,Seyer JM,Fried VA,Coughlin SR,Phillips DR,Jennings LK cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins. J Biol Chem 1991 266:10638 PubMed

8. Le Naour F,Prenant M,Francastel C,Rubinstein E,Uzan G,Boucheix C Transcriptional regulation of the human CD9 gene: characterization of the 5'-flanking region. Oncogene 1996 13:481 PubMed

9. Loffler S,Lottspeich F,Lanza F,Azorsa DO,ter Meulen V,Schneider-Schaulies J CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J Virol 1997 71:42 PubMed

10. Oritani K,Wu X,Medina K,Hudson J,Miyake K,Gimble JM,Burstein SA,Kincade PW Antibody ligation of CD9 modifies production of myeloid cells in long- term cultures. Blood 1996 87:2252 PubMed

11. Rubinstein E,Le Naour F,Billard M,Prenant M,Boucheix C CD9 antigen is an accessory subunit of the VLA integrin complexes. Eur J Immunol 1994 24:3005 PubMed

12. Rubinstein E,Le Naour F,Lagaudriere-Gesbert C,Billard M,Conjeaud H,Boucheix C CD9, CD63, CD81, and CD82 are components of a surface tetraspan network connected to HLA-DR and VLA integrins. Eur J Immunol 1996 26:2657 PubMed

13. Seehafer JG,Tang SC,Slupsky JR,Shaw AR The functional glycoprotein CD9 is variably acylated: localization of the variably acylated region to a membrane-associated peptide containing the binding site for the agonistic monoclonal antibody 50H.19. Biochim Biophys Acta 1988 957:399 PubMed

14. Shaw AR,Domanska A,Mak A,Gilchrist A,Dobler K,Visser L,Poppema S,Fliegel L,Letarte M,Willett BJ Ectopic expression of human and feline CD9 in a human B cell line confers beta 1 integrin-dependent motility on fibronectin and laminin substrates and enhanced tyrosine phosphorylation. J Biol Chem 1995 270:24092 PubMed

15. Tai XG,Yashiro Y,Abe R,Toyooka K,Wood CR,Morris J,Long A,Ono S,Kobayashi M,Hamaoka T,Neben S,Fujiwara H A role for CD9 molecules in T cell activation. J Exp Med 1996 184:753 PubMed

16. Willett BJ,Hosie MJ,Jarrett O,Neil JC Identification of a putative cellular receptor for feline immunodeficiency virus as the feline homologue of CD9. Immunology 1994 81:228 PubMed

WWW RESOURCES


* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov