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PROW: CD28
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PROW and IWHLDA present the GUIDE on:
CD28
Author: Miyuki Azuma
Reviewer: Peter S. Linsley
[GUIDE BAR]

ALTERNATE NAMES FOR CD28  

MAJOR LINKS FOR CD28  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD28   - No information

CELLULAR FUNCTION OF CD28  

DISEASE RELEVANCE OF CD28 AND FUNCTION OF CD28 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD28  

MOLECULAR STRUCTURE OF CD28  

MOLECULAR MASS OF CD28  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
T lymphocytes ~90 kDa ~44 kDa  
COS cells ~74 kDa ~39 kDa  

POST-TRANSCRIPTIONAL MODIFICATION OF CD28  

POST-TRANSLATIONAL MODIFICATION OF CD28  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD28   - No information

SUBSTRATES FOR CD28   - No information

ENZYMES WHICH MODIFY CD28   - No information

LIGANDS FOR CD28 AND MOLECULES ASSOCIATED WITH CD28  

MOLECULECOMMENT
CD80 The CD80 molecule is also a ligand for CTLA-4, which binds with high avidity
CD86 The CD86 molecule is also a ligand for CTLA-4, which binds with high avidity
PI3-kinase The cytoplasmic region of CD28 binds to this intracellular signaling molecule. The pYMNM motif binds to the SH2 domains of PI3-kinase and GRB-2
ITK The cytoplasmic region of CD28 binds to this intracellular signaling molecule
GRB-2-SOS (signaling complex) The cytoplasmic region of CD28 binds to this intracellular signaling molecule. The pYMNM motif binds to the SH2 domains of PI3-kinase and GRB-2

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD28  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD28  
REAGENTS
CD28-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
B-T3 (6T-040) Vermot Desroches C/ J Wijdenes  
B-L8 (6T-041) Vermot Desroches C/ J Wijdenes  
4B10 (6T-109) Patricia Roth Coulter  

SELECTION OF OTHER CD28-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
KOLT-2 K Okubo (Nichirei)  
9.3 JA Hansen  
Leu28 (L293) NL Warner (Becton Dickinson)  

 
SELECTED REFERENCES ON CD28  

REVIEWS

1. June CH,Bluestone JA,Nadler LM,Thompson CB The B7 and CD28 receptor families. Immunol Today 1994 15:321 PubMed

2. June CH,Ledbetter JA,Linsley PS,Thompson CB Role of the CD28 receptor in T-cell activation. Immunol Today 1990 11:211 PubMed

3. Linsley PS,Ledbetter JA The role of the CD28 receptor during T cell responses to antigen. Annu Rev Immunol 1993 11:191 PubMed

PRIMARY CITATIONS

4. Aruffo A,Seed B Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc Natl Acad Sci U S A 1987 84:8573 PubMed

5. Hansen JA, Martin PJ and Nowinski RC. 1980. Immunogenetics 10: 247

6. Ledbetter JA,Martin PJ,Spooner CE,Wofsy D,Tsu TT,Beatty PG,Gladstone P Antibodies to Tp67 and Tp44 augment and sustain proliferative responses of activated T cells. J Immunol 1985 135:2331 PubMed

7. Lee KP,Taylor C,Petryniak B,Turka LA,June CH,Thompson CB The genomic organization of the CD28 gene. Implications for the regulation of CD28 mRNA expression and heterogeneity. J Immunol 1990 145:344 PubMed

8. Shahinian A,Pfeffer K,Lee KP,Kundig TM,Kishihara K,Wakeham A,Kawai K,Ohashi PS,Thompson CB,Mak TW Differential T cell costimulatory requirements in CD28-deficient mice. Science 1993 261:609 PubMed

9. Weiss A,Manger B,Imboden J Synergy between the T3/antigen receptor complex and Tp44 in the activation of human T cells. J Immunol 1986 137:819 PubMed

WWW RESOURCES


* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov