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PROW: CD14
[PROW BAR]
PROW and IWHLDA present the GUIDE on:
CD14
Author: Sanna M. Goyert
Reviewer: Samuel D. Wright
[GUIDE BAR]

ALTERNATE NAMES FOR CD14  

MAJOR LINKS FOR CD14  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD14  

CELLULAR FUNCTION OF CD14  

DISEASE RELEVANCE OF CD14 AND FUNCTION OF CD14 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD14  

MOLECULAR STRUCTURE OF CD14  

MOLECULAR MASS OF CD14  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
Monocytes 53 kDa 55 kDa  

POST-TRANSCRIPTIONAL MODIFICATION OF CD14   - No information

POST-TRANSLATIONAL MODIFICATION OF CD14  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD14   - No information

SUBSTRATES FOR CD14   - No information

ENZYMES WHICH MODIFY CD14  

MOLECULECOMMENT
LBP (Lipopolysaccharide Binding Protein** LBP is a lipid transfer protein that moves monomers of LPS, phosphatidyl inositol, and other phospholipids to a binding site on CD14. (Hailman et al. 1994)**

LIGANDS FOR CD14 AND MOLECULES ASSOCIATED WITH CD14  

MOLECULECOMMENT
Endotoxin (lipopolysaccharide) Direct binding data indicate that each CD14 molecule binds 1-2 molecules of LPS. The binding of LPS to CD14 accelerated in the presence of LBP (Hailman et al. 1994)**
Lipoteichoic acid** In vitro binding studies show that lipoteichoic acid and phosphatidyl inositol bind to sCD14 (Yu et al. 1997). The physiological relevance of this binding has not yet been demonstrated
Phosphatidyl inositol** In vitro binding studies show that lipoteichoic acid and phosphatidyl inositol bind to sCD14 (Yu et al. 1997). The physiological relevance of this binding has not yet been demonstrated

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD14  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD14  
REAGENTS
CD14-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
ZCH-7-2F9 (M47) YM Tang, USA  
GP3 (MA3) A Bensussan, France  
4H6 (MA32) H Heyligen, Belgium  
4G7 (MA33) H Heyligen, Belgium  
4D11 (MA34) H Heyligen, Belgium  
2C6 (MA35) H Heyligen, Belgium  
HI221 (MA52) de Cheng Shen, China  
HI222 (MA53) de Cheng Shen, China  
RMO52 (MA62) A van Agthoven, France  
61D3 (M13) C Gregory, England  

SELECTION OF OTHER CD14-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
RPA-M1 (MR1) G Aversa, USA  
MoS39 (MR3) Goyert et al. 1986  
3C10** RM Steinman, USA  
60b**    

 
SELECTED REFERENCES ON CD14  

REVIEWS

PRIMARY CITATIONS

1. Bazil V and Strominger JL. Shedding as a mechanism of down-modulation of CD14 on stimulated human monocytes. J. Immunol. 1991 147:1567 PubMed

2. Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W and Hilgert I. Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur. J. Immunol. 1986 16:1583 PubMed

3. Bufler P, Stiegler G, Schuchmann M, Hess S, Kruger C, Stelter F, Eckerskorn C, Schutt C and Engelmann H. Soluble lipopolysaccharide receptor (CD14) is released via two different mechanisms from human monocytes and CD14 transfectants. Eur. J. Immunol. 1995 25:604 PubMed

4. Fearns C, Kravchenko VV, Ulevitch RJ and Loskutoff DJ. Murine CD14 gene expression in vivo: extramyeloid synthesis and regulation by lipopolysaccharide. J. Exp. Med. 1995 181:857 PubMed

5. Ferrero E and Goyert SM. Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14. Nucleic Acids Res. 1988 16:4173 PubMed

6. Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F and Le Beau MM. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science 1988 239:497 PubMed

7. Goyert SM, Ferrero EM, Seremetis SV, Winchester RJ, Silver J and Mattison AC. Biochemistry and expression of myelomonocytic antigens. J. Immunol. 1986 137:3909 PubMed

8. Hailman E, Lichenstein HS, Wurfel MM, Miller DS, Johnson DA, Kelley M, Busse LA, Zukowski MM and Wright SD. Lipopolysaccharide (LPS)-binding protein accelerates the binding of LPS to CD14. J. Exp. Med. 1994 179:269 ** PubMed

9. Haziot A, Chen S, Ferrero E, Low MG, Silber R and Goyert SM. The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage. J. Immunol. 1988 141:547 PubMed

10. Haziot A, Ferrero E, Kontgen F, Hijiya N, Yamamoto S, Silver J, Stewart CL and Goyert SM. Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity 1996 4:407 PubMed

11. Haziot A, Tsuberi BZ and Goyert SM. Neutrophil CD14: biochemical properties and role in the secretion of tumor necrosis factor-alpha in response to lipopolysaccharide. J. Immunol. 1993 150:5556 PubMed

12. Haziot, A., Katz, I., Jiao, D., Rong, G,-W., and Goyert, S.M. Functional epitopes of CD14. In Leukocyte Typing V: White cell differentiation antigens. (Eds. S. Schlossman, et al.) Oxford University Press, pp. 782-784,(1995)

13. Labeta MO, Durieux JJ, Fernandez N, Herrmann R and Ferrara P. Release from a human monocyte-like cell line of two different soluble forms of the lipopolysaccharide receptor, CD14. Eur. J. Immunol. 1993 23:2144 PubMed

14. Landmann R, Zimmerli W, Sansano S, Link S, Hahn A, Glauser MP and Calandra T. Increased circulating soluble CD14 is associated with high mortality in gram-negative septic shock. J. Infect. Dis. 1995 171:639 PubMed

15. Pugin J, Ulevitch RJ and Tobias PS. Activation of endothelial cells by endotoxin: direct versus indirect pathways and the role of CD14. Prog. Clin. Biol. Res. 1994 392:369 PubMed

16. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ and Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 1990 249:1431 ** PubMed

17. Wurfel MM, Hailman E and Wright SD. Soluble CD14 acts as a shuttle in the neutralization of lipopolysaccharide (LPS) by LPS-binding protein and reconstituted high density lipoprotein. J. Exp. Med. 1995 181:1743 ** PubMed

18. Yu B, Hailman E and Wright SD. Lipopolysaccharide binding protein and soluble CD14 catalyze exchange of phospholipids. J. Clin. Invest. 1997 99:315 ** PubMed

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Modified 10/14/99   mpr@mail.nih.gov