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PROW: CDw150
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PROW and IWHLDA present the GUIDE on:
CDw150
Author: Svetlana P. Sidorenko
Reviewer: Gordon J. Freeman
Link to additional info in FORUM
[GUIDE BAR]

OVERVIEW
MOLECULAR FAMILIES  

ALTERNATE NAMES  

  • SLAM [HUGO gene name]
  • IPO-3
  • Signaling Lymphocyte Activation Molecule

    MAJOR LINKS  

  • NCBI LocusLink Record: 6504
  • Mendelian Inheritance in Man (OMIM): 603492
  • AUTHOR'S ADDITIONAL INSIGHTS  

  • Important question 1. What are the ligands for surface and secreted forms?
  • Important question 2. What signal transduction pathways are triggered by CDw150 in T and B lymphocytes?
    SYSTEM VIEW
    DISEASE RELEVANCE / FUNCTION IN INTACT  
    - Unknown

    CELLULAR FUNCTION  

  • Costimulatory molecule on B lymphocytes and dendritic cells*
  • Binding of CDw150 to its ligand(s) enhances proliferation and Ig production by activated B cells. This makes CDW150 a receptor for a costimulatory signal, not a costimulatory molecule. The Cock's paper emphasizes the CDW150 signal as upregulating interferon-gamma production**

    CELLULAR EXPRESSION  

  • Thymocytes
  • CD45RO positive subpopulation of T lymphocytes
  • B lymphocytes
  • Dendritic cells
  • Endothelial cells
  • Upregulated after activation
    GENE
    TRANSCRIPTION REGULATING MOLECULES  
    - Unknown


    TRANSCRIPT
    POST-TRANSCRIPTIONAL MODIFICATION  
  • Three alternatively spliced forms were cloned: full length molecule, secreted form with deleted transmembrane region (amino acids 234-263), and form with modified truncated cytoplasmic tail
    PROTEIN
    BIOCHEMICAL ACTIVITY  
    - Unknown

    PROTEIN SEQUENCE ANNOTATION  

  • Single chain type I transmembrane molecule (335 amino acids with 27 amino acid leader sequence)
  • A 209 amino acid extracellular region composed of one V and one C2 Ig-like domains and has 8 potential N-glycosylation sites
  • Transmembrane region has 22 amino acids
  • Intracytoplasmic tail (77amino acids) has tyrosine and serine/threonine phosphorylation sites

    PROTEIN PHYSICAL PROPERTIES  

    CELL TYPEMW UNREDUCEDMW REDUCEDComment
    B lymphocytes 65-85 kDa 75-95 kDa Protein core 41 kDa, sialic acids 10 kDa
    T lymphocytes   70 kDa Protein core 40 kDa
    B lymphocytes 65-85 kDa 75-95 kDa  
    T lymphocytes   70 kDa  

    POST-TRANSLATIONAL MODIFICATION  

  • Extensive N-linked glycosylation (asparagine-linked), terminal sialic acid residues on oligosaccharide chains
  • Intracytoplasmic tail (77amino acids) has tyrosine and serine/threonine phosphorylation sites
  • Three of four tyrosine phosphorylation sites form potential binding site for SH2 domains
    MOLECULAR INTERACTIONS
    SUBSTRATES  
    - Unknown

    ENZYME FOR WHICH THIS IS A SUBSTRATE  

    MOLECULECOMMENT
    Tyrosine and serine/threonine kinases In B lymphoblastoid cell lines
    Tyrosine phosphatase (possible CD45) In B lymphoblastoid cell lines

    OTHER LIGANDS AND ASSOCIATED MOLECULES Link to additional info in FORUM  

    MOLECULECOMMENT
    CDw150 High affinity self ligand
    Tyrosine and serine/threonine kinases Associates in B cells
    Tyrosine phosphatase CD45 Associates in B cells
  • Crosslinking of ligand(s) enhance proliferation and Ig production by activated B cells
    REAGENTS
    MABS NEW  
    NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
    IPO-3   {Sidorenko_SP/Gluzman_DF.1992.NEOPL}

    MABS REFERENCE  

    NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
    A12   {Cocks_BG/Aversa_G.1995.NATUR}

    REFERENCES
    REVIEWS  

    PRIMARY CITATIONS  
    1. {Cocks_BG/Aversa_G.1995.NATUR}
    Cocks BG, Chang CC, Carballido JM, Yssel H, de Vries JE and Aversa G. 1995. A novel receptor involved in T-cell activation. Nature 376:260-263
    2. {Pinchouk_VG/Schlapatskaya_LN.1988.AR}
    Pinchouk VG, Sidorenko SP, Gluzman DF, Vetrova EP, Berdova AG and Schlapatskaya LN. 1988. Monoclonal antibodies IPO-3 and IPO-10 against human B cell differentiation antigens. Anticancer Res 8:1377-1380
    3. {Polacino_PS/Clark_EA.1996.JMP}
    Polacino PS, Pinchuk LM, Sidorenko SP and Clark EA. 1996. Immunodeficiency virus cDNA synthesis in resting T lymphocytes is regulated by T cell activation signals and dendritic cells. J Med Primatol 25:201-209
    4. {Punnonen_J/de_Vries_JE.1997.JEM}
    Punnonen J, Cocks BG, Carballido JM, Bennett B, Peterson D, Aversa G and de Vries JE. 1997. Soluble and membrane-bound forms of signaling lymphocytic activation molecule (SLAM) induce proliferation and Ig synthesis by activated human B lymphocytes. J Exp Med 185:993-1004
    5. {Sidorenko_SP/Clark_EA.1993.JI}
    Sidorenko SP and Clark EA. 1993. Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes. J Immunol 151:4614-4624
    6. {Sidorenko_SP/Gluzman_DF.1992.NEOPL}
    Sidorenko SP, Vetrova EP, Yurchenko OV, Berdova AG, Shlapatskaya LN and Gluzman DF. 1992. Monoclonal antibodies of IPO series against B cell differentiation antigens in leukemia and lymphoma immunophenotyping. Neoplasma 39:3-9

    WWW RESOURCES  


    * indicates amended by reviewer, ** indicates added by reviewer
    Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission
    Modified 01/25/00   mpr@mail.nih.gov