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PROW: CD45
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PROW and IWHLDA present the GUIDE on:
CD45
Authors: William A. Sewell; Margaret A. Cooley; Kenneth S. Katz
Reviewers: Matthew L. Thomas; A. Neil Barclay
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ALTERNATE NAMES FOR CD45  

MAJOR LINKS FOR CD45  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD45  

CELLULAR FUNCTION OF CD45  

DISEASE RELEVANCE OF CD45 AND FUNCTION OF CD45 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD45  

MOLECULAR STRUCTURE OF CD45  

MOLECULAR MASS OF CD45  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
Naive/resting T lymphocytes 210 kDa, 220 kDa    
Memory/activated T lymphocytes 180 kDa, 200 kDa    
B lymphocytes 220 kDa    
Monocytes 180 kDa, 200 kDa    
Granulocytes 180 kDa, 200 kDa    
NK cells 210 kDa, 220 kDa    
Dendritic cells 180 kDa, 200 kDa    

POST-TRANSCRIPTIONAL MODIFICATION OF CD45  

POST-TRANSLATIONAL MODIFICATION OF CD45  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD45   - No information

SUBSTRATES FOR CD45  

MOLECULECOMMENT
p56lck (Src-kinase) dephosphorylation of substrate induces or reduces enzymatic activity of the substrate*
p59fyn (Src-kinase) dephosphorylation of substrate induces or reduces enzymatic activity of the substrate*
Other src-kinases CD45 regulates srcs in other cell types. CD45 will dephosphorylate both the autophosphorylation and the negative phosphorylation site. Therefore, CD45 decreases src kinase activity in some circumstances**

ENZYMES WHICH MODIFY CD45   - No information

LIGANDS FOR CD45 AND MOLECULES ASSOCIATED WITH CD45  

MOLECULECOMMENT
Galectin-1 Also appears to be an important interacting molecule (Perillo et al. 1995)**
CD2 Associates with extracellular domain, significance controversial
CD3 Associates with extracellular domain, significance controversial
CD4 Associates with extracellular domain, significance controversial
LPAP (lymphocyte phosphatase-associated phosphoprotein)(CD45AP) CD45 non-covalently associates with LPAP, a 32k transmembrane protein of T and B lymphocytes. Association requires the transmembrane regions of both molecules

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD45  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD45  
REAGENTS
CD45-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
CBE.77 Delsol CD45
IMMU19.2 van Agthoven CD45
CLBT200.1 Connelly CD45
7E.12 Taskov CD45
CF10H5 Hadam CD45
HI151 Shen CD45
HI185 Shen CD45
ML2 Poppema CD45
DBB.42 Delsol CD45RA
LT45.M5 Filatov CD45RA
BIRMA12 McDonald CD45RA
HI115 Shen CD45RA
OTH74D4 Hadam CD45RA
WM76 Henniker CD45RB
MT3 Poppema CD45RB
MT4 Poppema CD45RB
MT5 Poppema CD45RB
OTH75E4 Hadam CD45RC
11G8 Vanlier /Voorn CD45RC

SELECTION OF OTHER CD45-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
Anti-HLe-1 Beverley 1980 CD45
UCHL1 Terry LA et al. 1988 CD45RO
2H4 Morimoto C et al. 1985 CD45RA
PD-7/26 Pulido R et al. 1988 CD45RB
RP1/12 Zapata J et al. 1994 CD45RC

 
SELECTED REFERENCES ON CD45  

REVIEWS

1. Barclay AN and McCall MN. CD45; from alloantigen to mapping of restricted epitopes using recombinant soluble CD45 isoforms. Biochem. Soc. Trans. 1992 20:161 PubMed

2. Trowbridge IS and Thomas ML. CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 1993 12:85 PubMed

PRIMARY CITATIONS

3. Beverley PCL. 1980. in Production and Use of Monoclonal Antibodies in Transplantation Immunology. In Transplantation and Clinical Immunology XI. pp 87-94, eds Touraine JL et al, Excerpta Medica Amsterdam.

4. Kishihara K, Penninger J, Wallace VA, Kundig TM, Kawai K, Wakeham A, Timms E, Pfeffer K, Ohashi PS, Thomas ML and et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell 1993 74:143 PubMed

5. Morimoto C, Letvin NL, Distaso JA, Aldrich WR and Schlossman SF. The isolation and characterization of the human suppressor inducer T cell subset. J. Immunol. 1985 134:1508 PubMed

6. Okumura M, Matthews RJ, Robb B, Litman GW, Bork P and Thomas ML. Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains. J. Immunol. 1996 157:1569 PubMed

7. Perillo NL, Pace KE, Seilhamer JJ and Baum LG. Apoptosis of T cells mediated by galectin-1. Nature 1995 378:736 PubMed

8. Pingel JT and Thomas ML. Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation. Cell 1989 58:1055 PubMed

9. Pulido R, Cebrian M, Acevedo A, de Landazuri MO and Sanchez-Madrid F. Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J. Immunol. 1988 140:3851 PubMed

10. Roach T, Slater S, Koval M, White L, McFarland EC, Okumura M, Thomas M and Brown E. CD45 regulates Src family member kinase activity associated with macrophage integrin-mediated adhesion. Curr. Biol. 1997 7:408 PubMed

11. Terry LA, Brown MH and Beverley PC. The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45. Immunology 1988 64:331 PubMed

12. Tonks NK, Charbonneau H, Diltz CD, Fischer EH and Walsh KA. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochemistry 1988 27:8695 PubMed

13. Zapata JM, Pulido R, Acevedo A, Sanchez-Madrid F and de Landazuri MO. Human CD45RC specificity. A novel marker for T cells at different maturation and activation stages. J. Immunol. 1994 152:3852 PubMed

WWW RESOURCES

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* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/15/99   mpr@mail.nih.gov