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PROW: CD34
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PROW and IWHLDA present the GUIDE on:
CD34
Authors: H. Nishio; J. Tada; N. Hashiyama; J. Hirn; J. Ingles-Esteven; Toshio Suda
Reviewers: Curt I. Civin; Mary Jo Fackler
Link to additional info in FORUM
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ALTERNATE NAMES FOR CD34  

MAJOR LINKS FOR CD34  


FUNCTION
BIOCHEMICAL ACTIVITY OF CD34   - No information

CELLULAR FUNCTION OF CD34 Link to additional info in FORUM  

DISEASE RELEVANCE OF CD34 AND FUNCTION OF CD34 IN INTACT ANIMAL  


STRUCTURE
MOLECULAR FAMILY FOR CD34  

MOLECULAR STRUCTURE OF CD34  

MOLECULAR MASS OF CD34  

CELL TYPEMW UNREDUCEDMW REDUCEDComment
Various cells 116 kDa predicted 40 kDa deduced   Although 116 kDa is the molecular weight as estimated by mobility of the naturally occurring glycoprotein, note that molecular mobility of CD34 is strongly influenced by its charge, mainly due to glycosylation. In fact, the amino acid sequence deduced from the human CD34 gene sequence predicts a polypeptide of only 40kDa

POST-TRANSCRIPTIONAL MODIFICATION OF CD34  

POST-TRANSLATIONAL MODIFICATION OF CD34  


MOLECULAR INTERACTIONS
PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD34  
MOLECULECOMMENT
myb Potential physiologic activation of CD34 has been shown to occur in CD34+ glioblastoma cell lines
myc c-myc is expressed in most proliferating cell types. The gene products play an essential role in normal cell growth and development
ets-2 Transcription factor can activate human CD34 transcription independently
mzf-1 Zinc finger protein that is up-regulated during myeloid differentiation, can bind to CD34 promotor
NC-3A A multiprotein complex can positively regulate the human CD34 promotor via the TCATTT motif, which can act as an enhancer

SUBSTRATES FOR CD34   - No information

ENZYMES WHICH MODIFY CD34   - No information

LIGANDS FOR CD34 AND MOLECULES ASSOCIATED WITH CD34  

MOLECULECOMMENT
L-selectin L-selectin is the lymphocyte homing receptor and binds to both GLYCAM-1 and CD34 from high vein endothelial cells in lymph nodes. However, L-selectin does not appear to bind vascular CD34 outside of high endothelial venules or to hematopoietic CD34

EXPRESSION
MAIN CELLULAR EXPRESSION OF CD34 Link to additional info in FORUM  
 
AUTHOR'S ADDITIONAL INSIGHTS ON CD34   - No information


REAGENTS
CD34-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP  
NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
ICO115 (MA2) Bryshnikov  
B-G25 (MA9) Clement  
B-H21 (MA10) Clement  
NU4A1 (MA42) Nakamura  
45.28 (MA46) Reisbach  
Birma-K3 (MA6) Broe  
B-F23 (MA8) Clement  
6A6 (MA54) Simmons  
7E10 (MA55) Simmons  
4H11 (MA58) Stockbauer  

SELECTION OF OTHER CD34-SPECIFIC REFERENCE MAB  

NAME(Workshop IDs)SOURCE or REFERENCECOMMENT
IMMU409 Hirn  
IMMU133 Hirn  
Qbend10 Jacob  
581 Gaudernack  
8G12 Warner  
My10 Lanier  

 
SELECTED REFERENCES ON CD34  

REVIEWS

1. Krause DS,Fackler MJ,Civin CI,May WS CD34: structure, biology, and clinical utility. Blood 1996 87:1 PubMed

2. Sutherland DR,Keating A The CD34 antigen: structure, biology, and potential clinical applications. J Hematother 1992 1:115 PubMed

PRIMARY CITATIONS

3. Baumheter S,Singer MS,Henzel W,Hemmerich S,Renz M,Rosen SD,Lasky LA Binding of L-selectin to the vascular sialomucin CD34. Science 1993 262:436 PubMed

4. Civin CI,Strauss LC,Brovall C,Fackler MJ,Schwartz JF,Shaper JH Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG- 1a cells. J Immunol 1984 133:157 PubMed

5. Fackler MJ,Krause DS,Smith OM,Civin CI,May WS Full-length but not truncated CD34 inhibits hematopoietic cell differentiation of M1 cells. Blood 1995 85:3040 PubMed

6. Gaudernack, G. and Egeland, T. Leucocyte typing (ed.Schlossman, S.F. et al), pp. 861-4. Oxford University Press, Oxford (1995)

7. Huyhn A,Dommergues M,Izac B,Croisille L,Katz A,Vainchenker W,Coulombel L Characterization of hematopoietic progenitors from human yolk sacs and embryos. Blood 1995 86:4474 PubMed

8. Lin G,Finger E,Gutierrez-Ramos JC Expression of CD34 in endothelial cells, hematopoietic progenitors and nervous cells in fetal and adult mouse tissues. Eur J Immunol 1995 25:1508 PubMed

9. Nakamura Y,Komano H,Nakauchi H Two alternative forms of cDNA encoding CD34. Exp Hematol 1993 21:236 PubMed

10. Perrotti D,Bellon T,Trotta R,Martinez R,Calabretta B A cell proliferation-dependent multiprotein complex NC-3A positively regulates the CD34 promoter via a TCATTT-containing element. Blood 1996 88:3336 PubMed

11. Sato N,Sawada K,Koizumi K,Tarumi T,Ieko M,Yasukouchi T,Yamaguchi M,Takahashi TA,Sekiguchi S,Koike T In vitro expansion of human peripheral blood CD34+ cells. Blood 1993 82:3600 PubMed

12. Satterthwaite AB,Burn TC,Le Beau MM,Tenen DG Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. Genomics 1992 12:788 PubMed

13. Suda J,Sudo T,Ito M,Ohno N,Yamaguchi Y,Suda T Two types of murine CD34 mRNA generated by alternative splicing. Blood 1992 79:2288 PubMed

14. Sutherland DR,Abdullah KM,Cyopick P,Mellors A Cleavage of the cell-surface O-sialoglycoproteins CD34, CD43, CD44, and CD45 by a novel glycoprotease from Pasteurella haemolytica. J Immunol 1992 148:1458 PubMed

15. Tavian M,Coulombel L,Luton D,Clemente HS,Dieterlen-Lievre F,Peault B Aorta-associated CD34+ hematopoietic cells in the early human embryo. Blood 1996 87:67 PubMed

16. Tindle RW,Nichols RA,Chan L,Campana D,Catovsky D,Birnie GD A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non- T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrow. Leuk Res 1985 9:1 PubMed

WWW RESOURCES


* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov