Restriction Endonucleases
>
Afe I
Afe I
Recognition site:
AGC
▼
GCT
TCG
▲
CGA
Activity, NEB:
NEBuffer 1
NEBuffer 2
NEBuffer 3
NEBuffer 4
25%
50%
25%
100%
Recommended buffer:
NEB:
SEBuffer Y
Incubation temperature:
37°C
Heat Inactivation:
65°C for 20 minutes.
Links:
Afe I, NEB
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