Sac I
| |
| |
| | Recognition site:
| | | GAGCT▼C
C▲TCGAG | | | | | Activity, Fermentas:
| | |
| Blue+ |
Green+ |
Orange+ |
Red+ |
Yellow+ 1x |
Yellow+ 2x |
| | 50-100% |
20-50% |
0-20% |
0-20% |
50-100% |
20-50% |
| | | |
| | Activity, NEB:
| | |
| NEBuffer 1 |
NEBuffer 2 |
NEBuffer 3 |
NEBuffer 4 |
|
| 100% |
50% |
10% |
100% |
| | |
| | Activity, Promega:
| | |
| A |
B |
C |
D |
J |
MULTI-CORE™ |
|
| 75–100% |
25–50% |
25–50% |
<10% |
100% |
100% |
| | |
| | Recommended buffer: | | |
| Fermentas: | Buffer Sac I+ |
| NEB: | NEBuffer 1 + BSA 10 mM |
| Promega: | Buffer J |
| | |
| | Incubation temperature: | | |
37°C | | |
| | Heat Inactivation: | | |
65°C for 20 minutes. |
| |
| | Links:
| | |
Sac I, Fermentas
Sac I, NEB
Promega |
| |
|
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| |
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