Sph I
| |
| |
| | Recognition site:
| | | GCATG▼C
C▲GTACG | | | | |
| | Activity, NEB:
| | |
| NEBuffer 1 |
NEBuffer 2 |
NEBuffer 3 |
NEBuffer 4 |
|
| 100% |
100% |
50% |
100% |
| | |
| | Activity, Promega:
| | |
| A |
B |
C |
D |
K |
MULTI-CORE™ |
|
| 75–100% |
75–100% |
100% |
75–100% |
100% |
10–25% |
| | |
| | Recommended buffer: | | |
| NEB: | NEBuffer 2 |
| Promega: | Buffer K |
| | |
| | Incubation temperature: | | |
37°C | | |
| | Heat Inactivation: | | |
65°C for 20 minutes. |
| |
| | Links:
| | |
Sph I, NEB
Promega |
| |
|
|
| |
|
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