Primer Name

Sequence

Melting Tm
(AT=2,CG=4)

Melting Tm
Nearest Neighbor

KS

5’- CGAGGTCGACGGTATCG

56 °C

57 °C

M13 (-20) (Universal)

5’-GTAAAACGACGGCCAGT

52 °C

55 °C

M13 (-40)

5’-GTTTTCCCAGTCACGAC

52 °C

53 °C

M13 forward

5'-GTTTTCCCAGTCACGACGTTG

64 °C

59 °C

M13 reverse

5'-TGAGCGGATAACAATTTCACACAG

68 °C

58 °C

M13 Reverse

5’-CAGGAAACAGCTATGAC

50 °C

50 °C

M13 Reverse (-40)

5’-GTTGTGTGGAATTGTG

46 °C

48 °C

Neomycin forward

5'-AGGATCTCCTGTCATCTCACCTTGCTCCTG

92 °C

66 °C

Neomycin reverse

5'-AAGAACTCGTCAAGAAGGCGATAGAAGGCG

90 °C

65 °C

SK

5’- TCTAGAACTAGTGGATC

48 °C

46 °C

Sp6

5’- CATACGATTTAGGTGACACTATAG

66 °C

53 °C

T3

5'-CGAAATTAACCCTCACTAAAGGGA

68 °C

57 °C

T3

5’-ATTAACCCTCACTAAAG

46 °C

45 °C

T7

5'-GTAATACGACTACTATAGGG

56 °C

48 °C

T7 Reverse

5’-GCTAGTTATTGCTCAGCGG

58 °C

56 °C

l gt11 Forward

5’-GGTGGCGACGACTCCTGGAGCCCG

84 °C

71 °C

l gt11 Reverse

5’-TTGACACCAGACCAACTGGTAATG

70 °C

59 °C