Protease

Classification

Cleavage sites

Carboxypeptidase B

Zn-metalloprotease

C-terminal basic amino acids Lys and Arg

Endoprotease Glu-C (V8)

Ser-protease

Carboxylic side of Glu (and Asp in phosphate buffer, pH 7.8)

Endoproteinase Arg-C

Cys-protease

Carboxylic side of Arg

Endoproteinase Asp-N

Metalloprotease

Amino side of Asp and Cys

Endoproteinase Lys-C

Ser-protease

Carboxylic side of Lys

Factor Xa

Ser-endopeptidase

Ile-Glu-Gly-Arg^-X

Papain

Cys-endopeptidase

Bonds involving Arg, Lys, Glu, His, Gly, and Tyr.

Thrombin

endoprotease

Arg-Gly

Trypsin

Ser-endopeptidase

Carboxylic side of the basic amino acid Arg and Lys