Heat inactivation of serum inactivates heat-labile complement components and may also reduce the potential for mycoplasma contamination. Heat inactivation may not be necessary and may be detrimental to the growth promoting capacity of serum.

1. Thaw serum slowly to 37 °C.

2. Agitate the serum bottle to mix the contents. Set it into the 56 °C water bath so that the water level is just above the serum level.

3. Set a timer for 30 minutes. Swirl the serum every 5 to 10 minutes.

4. Cool the serum on ice.