1. Melt 100 ml 2% agar/LB.

2. Disperse to 5 ml screw-cap glass bottles. Fill 2/3 of the bottle.

3. Cap loosely, sterilize by autoclaving.

4. Wait until the agar sets completely.

5. Take a sterile inoculating loop, pick a colony (or dip into the culture). Pierce the agar to the bottom of the bottle several times.

6. Cap tightly and store at room temperature in dark.

7. For un-used stabs, cap tightly and store at room temperature.