Hyperscreen and Kodak BioMax MS intensifying screens
1. In a dark room, place a film on top of an intensifying screen,
and place sample on top of the film. The phosphor (calcium tungstate) side of the intensifying
screen must face the film and sample. The beta emitters hit the phosphor to
produce light.
2. Place the sandwich in a cassette and for optimal result keep
the cassette in a -70 °C freezer.
3. Using intensifying screen may reduce resolution. Intensifying screen
is useful for high energy radioisotope (32P
and 125I), but may not help for weak and medium energy
radioisotopes (3H, 14C,
33P, and 35S) since their
energy is too low to penetrate the film.
4. For best result, use a double sided film when using intensifying screen.
Kodak BioMax TranScreen
1. Kodak BioMax TranScreen intensifying screen captures the beta particles
prior to their being exposed to the film. The BioMax TranScreen then converts the energy
from the beta particles to photons. The BioMax TranScreen needs to be
sandwiched between the film and the sample.
2. Place the sandwich in a cassette and for optimal result keep
the cassette in a -70 °C freezer.
3. Using intensifying screen may reduce resolution. The BioMax TranScreen intensifying screen
can be used for high energy radioisotope (32P
and 125I) as well as for weak and medium energy
radioisotopes (3H, 14C,
33P, and 35S).
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