Edited by Chang Zhu
1. Make a Bradford solution:
| Coomassie Blue G-250 |
100 mg |
| 95% ethanol |
50 ml |
| 85% (w/v) phosphoric acid |
100 ml |
| ddH2O |
750 ml |
2. Using a 96-well plate, add
0, 2, 4, 6, 10, 15 and 20 µg of BSA (1 mg/ml) to 7 wells.
3. Add up to 20 µl samples.
4. Add 40 µl Bradford solution to wells containing BSA or samples.
5. Add ddH2O to bring the final volume to
200 µl.
6. Read absorbance at 595 nm.
9. Make a standard curve using the BSA reading and estimate the protein
concentration in the sample.
Note: Bradford assay will not be accurate for samples containing high
concentration of detergent.
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