1. Run a protein polyacrylamide gel.

2. Submerge the gel in the Commassie blue stain solution. Use just enough to completely submerge the polyacrylamide gel. Stain for 1-4 hours or overnight at room temperature with gentle shake.

Coomassie blue stain solution can be reused for serveral times.

3. Destaining in several changes of de-staining solution until protein bands are clear. Put a small sponge in the destaining solution to absorb coomassie blue dye particles.

4. Gel can be photographed under white light, stored in the destaining solution, or dried.