ATP (adenosine triphosphate 10 mM)
| disodium ATP | 0.055 | gram (g) | | Sterile ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: aliquot and store at -20 degree |
Acrylamide/Bisacrylamide (19:1, 40%)
| Acrylamide | 380 | gram (g) | | N,N-Methylene-bisacrylamide | 20 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: filter and store protected from light at 4 degree |
Acrylamide/Bisacrylamide (37.5:1, 30%)
| acrylamide | 300 | gram (g) | | bisacrylamide | 8 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: filter and store protected from light at 4 degree |
Agarose gel loading dye (10X)
Alkaline lysis solution (NaOH/SDS)
| 1N NaOH | 20 | millilitre (ml) | | 10% SDS | 10 | millilitre (ml) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Ammonium acetate (5M)
| NH4OAc | 385.4 | gram (g) | | ddH2O to | 500 | millilitre (ml) | | Total volume | 500 | millilitre (ml) | | Note: filter to sterilize |
Ammonium acetate (8.0M)
| NH4OAc | 61.7 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Ammonium acetate (9.5M)
| NH4OAc | 72.3 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Ammonium persulfate (10%)
| Ammonium persulfate | 1 | gram (g) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) |
Ampicillin (50 mg/ml)
| Ampicillin | 0.5 | gram (g) | | Sterile ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: aliquot and store at -20 degree |
BME (beta-mercaptoethanl 0.5M)
| beta-mercaptoethanl | 3.5 | millilitre (ml) | | ddH2O | 96.5 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
BME (beta-mercaptoethanl 1M)
| beta-mercaptoethanl | 6.98 | millilitre (ml) | | ddH2O | 93 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
BSA (1mg/ml)
| BSA | 5 | milligram (mg) | | Sterile ddH2O to | 5 | millilitre (ml) | | Total volume | 5 | millilitre (ml) | | Note: aliquot and store at -20 degree |
Calcium chloride (CaCl2)
| CaCl2*2H2O | 1.48 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave to sterilize and store at 4degree |
Chloramphenicol (25 mg/ml)
| Chloramphenicol | 250 | milligram (mg) | | Ethanol (100%) | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: dissolve in absolute ethanol, aliquot and store at -20 degree |
DTT (Dithiothreitol 1M)
| DTT | 1.54 | gram (g) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: aliquot and store at -20 degree |
Denhart Solution (100X)
| BSA | 2 | gram (g) | | Ficol 400,000 | 2 | gram (g) | | Polyvinylpyrrolidone 40,000 | 2 | gram (g) | | SSPE (20X) | 15 | millilitre (ml) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: store at -20 degree |
EDTA (0.5M pH8.0)
| Na2EDTA | 186.1 | gram (g) | | ddH2O to | 500 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: Dissolve in approx. 400 ml ddH2O, adjust pH to 8.0 with 10 N NaOH, and adjust to 1 liter final volume with distilled water. Autoclave to sterilize |
EtBr (ethidium bromide 5 mg/ml)
| Ethidium bromide | 500 | milligram (mg) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Ethonal (70%)
| 95% Ethonal | 74 | millilitre (ml) | | ddH2O | 26 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Gelatin (2%)
| gelatin | 2 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave and store at room temperature. |
Glucose (1M)
| glucose | 18 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: filter to sterilize |
Glucose (20%)
| Glucose | 20 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: filter sterilize |
Glycerol (Sterile)
| Note: autoclave to sterilize. |
Guanidine (6M pH6.4)
| Guanidine-HCl | 573.18 | gram (g) | | 1M Tris-HCl (pH7.6) | 50 | millilitre (ml) | | 0.5 M EDTA | 40 | millilitre (ml) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
HEPES (1M pH7.5)
| HEPES | 23.83 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: adjust pH to 7.5 with potassium hydroxide (KOH) and store at 4 degree |
Hygromycin B (100 mg/ml)
| Hygromycin | 1 | gram (g) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: filter sterilize, aliquot and store at -20 degree |
IPTG (20mg/ml)
| IPTG | 200 | milligram (mg) | | ddH2O | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: filter sterilize, aliquot and store at -20 degree |
IPTG (isopropyl b-D-thiogalactopyranoside 25 mg/ml)
| IPTG | 250 | milligram (mg) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) |
Isocitrate (1M)
| Na3isocitrate-2H2O | 29.41 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
KCl (1M)
| KCl | 7.5 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave to sterilize |
Kanamycin (25mg/ml)
| Kanamycin sulfate | 0.25 | gram (g) | | Sterile ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: aliquot and store at -20 degree |
LB medium
| Bacto-Tryptone | 10 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NaCl | 10 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: adjust pH to 7.0 and autoclave to sterilize |
LB plates
| Bacto-Tryptone | 10 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NaCl | 10 | gram (g) | | Bacto-agar | 15 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave to sterilize, cool to 55 degree, add antibiotic if desired, and pour into sterile petri dishes |
Lambda plates
| Bacto-tryptone | 10 | gram (g) | | Bacto-agar | 15 | gram (g) | | NaCl | 2.5 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave to sterilize and pour into sterile petri dishes |
Lambda top agar
| Bacto-tryptone | 10 | gram (g) | | Bacto-agar | 10 | gram (g) | | NaCl | 5 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave to sterilize |
M9 medium
| Na2HPO4 | 6 | gram (g) | | KH2PO4 | 3 | gram (g) | | NaCl | 0.5 | gram (g) | | NH4Cl | 1 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave. Add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl) before use. |
MOPS (1M)
| MOPS | 20.9 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: Dissolve in 80 ml ddH2O, adjust pH to 7.5 with 1 N NaOH, and bring volume to 100 ml with ddH2O |
Magnesium chloride (1M)
| MgCl2*6H2O | 20.3 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave to sterilize |
Magnesium sulfate (1M)
| MgSO4 | 12 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave to sterilize |
Manganese chloride (1M)
| MnCl2 | 1.98 | gram (g) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: store protected from light |
NYZ agar
| NaCl | 5 | gram (g) | | MgSO4*7H2O | 2 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NZ amine (casein hydrolysate) | 10 | gram (g) | | Bacto-agar | 15 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
NYZ top agarose
| NYZ Broth | 1 | litre (l) | | agarose | 7 | gram (g) | | Total volume | 1 | litre (l) | | Note: autoclave |
NZY Broth
| NaCl | 5 | gram (g) | | MgSO4*7H2O | 2 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NZ amine (casein hydrolysate) | 10 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: adjust pH to 7.5 and autoclave |
Neutral Buffered Formalin
| Formalin (40% formadehyde) | 100 | millilitre (ml) | | Sodium phosphate, monobasic, monohydrate | 4 | gram (g) | | Sodium phosphate, dibasic, anhydrous | 6.5 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
P1 (15 mM Tris pH8 10mM EDTA plasmid prep)
| 1M Tris-HCl (pH 8.0) | 1.5 | millilitre (ml) | | 0.5M EDTA | 2 | millilitre (ml) | | ddH2O to | 96.5 | millilitre (ml) | | Rnase A | 10 | milligram (mg) | | Total volume | 100 | millilitre (ml) | | Note: filter sterilize, or autoclave and add Rnase A before use. |
P2 (0.2N NaOH 1% SDS plasmid prep)
| 10N NaOH | 2 | millilitre (ml) | | 10% SDS | 10 | millilitre (ml) | | ddH2O | 88 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: autoclave to sterilize |
P3 (3M KOAc pH 5.5 plasmid prep)
| KOAc | 54 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: dissolve in 80 ml of ddH2O, adjust to pH5.5 with glacial acetic acid and add ddH2O to 100 millilitre. Autoclave to sterilize |
Phenol (TE-saturated)
| Note: add an equal volume of TE (pH7.5-8.0) to phenol, mix well, allow phases to allow phases to separate, remove and discard upper (aqueous) phase. Repeat until the pH of the aqueous phase is between 7.5-8.0 (store protected from light at 4 degree) |
chloroform/isoamylalcohol(25:25:1)>Phenol/chloroform/isoamyl alcohol (25:25:1)
| TE-saturated phenol | 100 | millilitre (ml) | | chloroform | 100 | millilitre (ml) | | isoamyl alcohol | 4 | millilitre (ml) | | Total volume | 204 | millilitre (ml) | | Note: store protected from light at 4 degree |
Polyacrylamide Gel loading dye
| xylene cyanole FF | 3 | gram (g) | | bromophenol blue | 3 | gram (g) | | 0.5 M EDTA | 0.2 | millilitre (ml) | | deionized formamide | 10 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: for sequencing gel |
Potassium acetate (7.5M)
| KOAc | 736 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
Proteinase K (10mg/ml)
| Proteinase K | 0.01 | gram (g) | | TE | 1 | millilitre (ml) | | Total volume | 1 | millilitre (ml) | | Note: aliquot and store at -20 degree |
SM
| NaCl | 5.8 | gram (g) | | MgSO4*7H2O | 2 | gram (g) | | 1M Tris-HCl pH7.5 | 50 | millilitre (ml) | | Gelatin (2%) | 5 | millilitre (ml) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave and store at room temperature. |
SOB medium
| Bacto-tryptone | 20 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NaCl | 0.5 | gram (g) | | 1M KCl | 2.5 | millilitre (ml) | | ddH2O to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: adjust pH to 7.0 with 10N NaOH, autoclave to sterilize, and add 10 ml of 1 M MgCl2 before use |
SOC medium
| Bacto-tryptone | 20 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | NaCl | 0.5 | gram (g) | | 1M KCl | 2.5 | millilitre (ml) | | ddH2O to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: adjust pH to 7.0 with 10N NaOH, autoclave to sterilize, add 20 ml of sterile 1 M glucose immediately before use |
SSC (20X)
| NaCl | 175.3 | gram (g) | | Sodium citrate | 88.22 | gram (g) | | ddH2O to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: dissolve in 800 ml of ddH2O, adjust to pH7 with HCl and add ddH2O to 1 litre |
SSPE (20X)
| NaCl | 310.4 | gram (g) | | 1M phosphate buffer | 200 | millilitre (ml) | | 0.5 M EDTA | 40 | millilitre (ml) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave and store at room temperature. |
Sodium Acetate (2M)
| NaOAc*3H2O | 27.22 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Sodium Acetate (3M pH4.8)
| NaOAc*3H2O | 40.8 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: dissolve in 80 ml of ddH2O, adjust to pH4.8 with glacial acetic acid and add ddH2O to 100 millilitre. Filter to sterilize |
Sodium Acetate (3M pH5.2)
| NaOAc*3H2O | 40.8 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: dissolve in 80 ml of ddH2O, adjust to pH5.2 with glacial acetic acid and add ddH2O to 100 millilitre. Filter to sterilize |
Sodium chloride (3M)
| NaCl | 17.5 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Sodium chloride (5M)
| NaCl | 292.2 | gram (g) | | ddH2O to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: autoclave to sterilize |
Sodium hydroxide (10N)
| NaOH | 40 | gram (g) | | ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Sodium hydroxide (1N)
| 10 N NaOH | 10 | millilitre (ml) | | ddH2O | 90 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Sodium phosphate (pH6.7)
Spermidine (0.1M)
| Spermidine | 0.255 | gram (g) | | ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: store at -20 degree |
T-TYN agar
| T-TYN broth | 1 | litre (l) | | Bacto-agar | 15 | gram (g) | | Total volume | 1 | litre (l) | | Note: autoclave. |
T-TYN broth
| Bacto-yeast extract | 5 | gram (g) | | Bacto-tryptone | 10 | gram (g) | | NaCl | 5 | gram (g) | | 1M Tris-HCl pH7.2 | 10 | millilitre (ml) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: adjust pH to 7.2. autoclave |
TBE (10X)
| Tris base | 216 | gram (g) | | Boric Acid | 110 | gram (g) | | EDTA | 16.6 | gram (g) | | Water to | 2 | litre (l) | | Total volume | 2 | litre (l) |
TE Buffer (100:1)
| 1 M Tris-HCl (pH 8.0) | 1 | millilitre (ml) | | 0.5 M EDTA | 0.02 | millilitre (ml) | | Water to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
TE Buffer (10:1)
| 1 M Tris-HCl (pH 8.0) | 1 | millilitre (ml) | | 0.5 M EDTA | 0.2 | millilitre (ml) | | Water to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) |
Tetracycline (10 mg/ml)
| Tetracycline | 1 | gram (g) | | Ethanol (100%) | 50 | millilitre (ml) | | Sterile ddH2O to | 100 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: dissolve in 50% ethonal, aliquot and store protected from light at -20 degree |
Tris-HCl (1M)
| Tris base | 121.1 | gram (g) | | ddH2O to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: dissolve in 800 ml of ddH2O, adjust to desired pH with concentrated HCl and add ddH2O to 1 litre |
Tris-HCl (2M)
| Tris base | 242.2 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: dissolve in 800 ml of ddH2O, adjust to desired pH with concentrated HCl and add ddH2O to 1 litre |
Tris-acetate-EDTA buffer (50x TAE)
| Tris-HCl | 240 | gram (g) | | Glacial acetic acid | 57.1 | millilitre (ml) | | 0.5 M EDTA | 10 | millilitre (ml) | | Water to | 1000 | millilitre (ml) | | Total volume | 1000 | millilitre (ml) | | Note: Store at room temperature |
Tris-glycine Native-Polyacrylamide Gel loading buffer (2X)
| 1M Tris-HCl (phH6.8) | 1.6 | millilitre (ml) | | Glycerol (100%) | 2 | millilitre (ml) | | B-mercaptoethanol | 1 | millilitre (ml) | | Bromophenol blue | 4 | milligram (mg) | | ddH2O | 5.4 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: store at 4 degree |
Tris-glycine Native-Polyacrylamide Gel running buffer (10X)
| Tris base | 30.3 | gram (g) | | Glycine | 144 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
Tris-glycine SDS-Polyacrylamide Gel (10% Separating)
| 30% Polyacrylamide | 33.3 | millilitre (ml) | | 1.5M Tris(pH8.8) | 25 | millilitre (ml) | | Ammonium persulfate (10%) | 1 | millilitre (ml) | | SDS (10%) | 1 | millilitre (ml) | | TEMED | 0.04 | millilitre (ml) | | dH2O | 39.7 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel (12% Separating)
| 30% Polyacrylamide | 40 | millilitre (ml) | | 1.5M Tris(pH8.8) | 25 | millilitre (ml) | | Ammonium persulfate (10%) | 1 | millilitre (ml) | | SDS (10%) | 1 | millilitre (ml) | | TEMED | 0.04 | millilitre (ml) | | dH2O | 33 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel (15% Separating)
| 30% Polyacrylamide | 50 | millilitre (ml) | | 1.5M Tris(pH8.8) | 25 | millilitre (ml) | | Ammonium persulfate (10%) | 1 | millilitre (ml) | | SDS (10%) | 1 | millilitre (ml) | | TEMED | 0.04 | millilitre (ml) | | dH2O | 23 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel (18% Separating)
| 30% Polyacrylamide | 60 | millilitre (ml) | | 1.5M Tris(pH8.8) | 25 | millilitre (ml) | | Ammonium persulfate (10%) | 1 | millilitre (ml) | | SDS (10%) | 1 | millilitre (ml) | | TEMED | 0.04 | millilitre (ml) | | dH2O | 13 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel (5% Stacking)
| 30% Polyacrylamide | 1.7 | millilitre (ml) | | 1M Tris(pH6.8) | 1.25 | millilitre (ml) | | Ammonium persulfate (10%) | 0.1 | millilitre (ml) | | SDS (10%) | 0.1 | millilitre (ml) | | TEMED | 0.01 | millilitre (ml) | | dH2O | 6.8 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel (8% Separating)
| 30% Polyacrylamide | 26.7 | millilitre (ml) | | 1.5M Tris(pH8.8) | 25 | millilitre (ml) | | Ammonium persulfate (10%) | 1 | millilitre (ml) | | SDS (10%) | 1 | millilitre (ml) | | TEMED | 0.06 | millilitre (ml) | | dH2O | 46.3 | millilitre (ml) | | Total volume | 100 | millilitre (ml) | | Note: use immediately |
Tris-glycine SDS-Polyacrylamide Gel loading buffer (2X)
| 1M Tris-HCl (phH6.8) | 1.6 | millilitre (ml) | | 10% SDS | 4 | millilitre (ml) | | Glycerol (100%) | 2 | millilitre (ml) | | B-mercaptoethanol | 1 | millilitre (ml) | | Bromophenol blue | 4 | milligram (mg) | | ddH2O | 1.4 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: store at 4 degree |
Tris-glycine SDS-Polyacrylamide Gel loading buffer (3X)
| 1M Tris-HCl (phH6.8) | 2.4 | millilitre (ml) | | 20% SDS | 3 | millilitre (ml) | | Glycerol (100%) | 3 | millilitre (ml) | | B-mercaptoethanol | 1.6 | millilitre (ml) | | Bromophenol blue | 6 | milligram (mg) | | Total volume | 10 | millilitre (ml) | | Note: store at 4 degree |
Tris-glycine SDS-Polyacrylamide Gel running buffer (10X)
| Tris base | 30.3 | gram (g) | | Glycine | 144 | gram (g) | | SDS | 10 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) |
Trypsin/EDTA (0.025% Trypsin 0.01% EDTA)
| Trypsin | 125 | milligram (mg) | | EDTA | 50 | milligram (mg) | | HBSS | 500 | millilitre (ml) | | Total volume | 500 | millilitre (ml) | | Note: filter sterilize, aliquot and store at -20 degree |
Trypsin/EDTA (0.05% Trypsin 0.02% EDTA)
| Trypsin | 250 | milligram (mg) | | EDTA | 100 | milligram (mg) | | HBSS | 500 | millilitre (ml) | | Total volume | 500 | millilitre (ml) | | Note: filter sterilize, aliquot and store at -20 degree |
Trypsin/EDTA (0.25% Trypsin 0.1% EDTA)
| Trypsin | 1250 | milligram (mg) | | EDTA | 500 | milligram (mg) | | HBSS | 500 | millilitre (ml) | | Total volume | 500 | millilitre (ml) | | Note: filter sterilize, aliquot and store at -20 degree |
X-gal (20 mg/ml)
| X-gal (5-bromo-4-chloro-3-indolyl b-D-galactopyranoside) | 0.2 | gram (g) | | DMF (dimethylformamide) | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) | | Note: aliquot and store protected from light at -20 degree |
YEPD medium
| YPD media | 1 | litre (l) | | Total volume | 1 | litre (l) |
YP medium
| Bacto-yeast extract | 10 | gram (g) | | Bacto-peptone | 20 | gram (g) | | ddH2O to | 900 | millilitre (ml) | | Total volume | 900 | millilitre (ml) | | Note: autoclave |
YPD medium
| Bacto-yeast extract | 10 | gram (g) | | Bacto-peptone | 20 | gram (g) | | ddH2O to | 900 | millilitre (ml) | | 20% dextrose | 100 | millilitre (ml) | | Total volume | 1 | litre (l) | | Note: autoclave 20% dextrose separately from the rest, combine and pour plates. |
YPD plates
| Bacto-yeast extract | 10 | gram (g) | | Bacto-peptone | 20 | gram (g) | | Bacto-agar | 20 | gram (g) | | ddH2O to | 900 | millilitre (ml) | | 20% dextrose | 100 | millilitre (ml) | | Total volume | 1 | litre (l) | | Note: autoclave 20% dextrose separately from the rest, combine and pour plates. |
YPG plates
| Bacto-yeast extract | 10 | gram (g) | | Bacto-peptone | 20 | gram (g) | | Bacto-agar | 20 | gram (g) | | ddH2O to | 600 | millilitre (ml) | | 7.5% glycerol | 400 | millilitre (ml) | | Total volume | 1 | litre (l) | | Note: autoclave 7.5% glycerol separately from the rest, combine and pour plates. |
YT medium (2X)
| Bacto-tryptone | 16 | gram (g) | | Bacto-yeast extract | 10 | gram (g) | | NaCl | 5 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave to sterilize |
YT plates
| Bacto-tryptone | 16 | gram (g) | | Bacto-yeast extract | 10 | gram (g) | | Bacto-agar | 16 | gram (g) | | NaCl | 5 | gram (g) | | ddH2O to | 1 | litre (l) | | Total volume | 1 | litre (l) | | Note: autoclave to sterilize, cool to 65 degree, add antibiotic if desired, and pour into sterile petri dishes |
YT top soft agar
| Bacto-tryptone | 8 | gram (g) | | Bacto-yeast extract | 5 | gram (g) | | Bacto-agar | 3.5 | gram (g) | | NaCl | 2.5 | gram (g) | | ddH2O to | 0.5 | litre (l) | | Total volume | 0.5 | litre (l) | | Note: Heat to melt agar. Distribute 50 ml aliquots in 100 ml dilution bottles; autoclave to sterilize. |
dNTP (10 mM)
| dATP (100 mM) | 0.1 | millilitre (ml) | | dCTP (100 mM) | 0.1 | millilitre (ml) | | dGTP (100 mM) | 0.1 | millilitre (ml) | | dTTP (100 mM) | 0.1 | millilitre (ml) | | ddH2O | 0.6 | millilitre (ml) | | Total volume | 1 | millilitre (ml) | | Note: aliquot and store at -20 degree |
rATP (adenosine triphosphate 100mM)
| dipotassium ATP | 619 | milligram (mg) | | Sterile ddH2O to | 10 | millilitre (ml) | | Total volume | 10 | millilitre (ml) |
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