Components and Supplementation of Various Culture Media





Abbreviations:	DMEM = Dulbecco's Modified Eagle's Medium

	FCS = Fetal Calf (or Bovine) Serum

	GPS = Glutamine-Penicillin-Streptomycin

	BME = b-Mercaptoethanol

	DMSO = Dimethylsulfoxide

	HEPES = [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] 

        Buffer

	NEAA = Non-essential Amino Acids

	N = Nucleosides





Feeder/STO Medium



	DMEM

	7% FCS

	1X GPS





M15 (ES Cell Growth Medium)



	DMEM

	15% FCS

	1X GPS

	1X BME





2X Freezing Medium



	This should be freshly prepared for each use.  Wear gloves when 

working with DMSO.



	60% DMEM

	20% FCS

	20% DMSO





Injection Medium



	DMEM

	5% FCS

	20 mM HEPES pH 7.2-7.4



Embryo Culture Medium



	DMEM

	20% FCS

	1X GPS

	1X BME

	1X NEAA

	1X N