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| Resources: Fluorophore Table |
Dye |
Absorbance Wavelength |
Emission Wavelength |
Visible color |
|
Hydroxycoumarin |
325 |
386 |
blue |
|
methoxycoumarin |
360 |
410 |
blue |
|
Alexa fluor |
345 |
442 |
blue |
|
aminocoumarin |
350 |
445 |
blue |
|
Cy2 |
490 |
510 |
green (dark) |
|
FAM |
495 |
516 |
green (dark) |
|
Alexa fluor 488 |
494 |
517 |
green (light) |
|
Fluorescein FITC |
495 |
518 |
green (light) |
|
Alexa fluor 430 |
430 |
545 |
green (light) |
|
Alexa fluor 532 |
530 |
555 |
green (light) |
|
HEX |
535 |
556 |
green (light) |
|
Cy3 |
550 |
570 |
yellow |
|
TRITC |
547 |
572 |
yellow |
|
Alexa fluor 546 |
556 |
573 |
yellow |
|
Alexa fluor 555 |
556 |
573 |
yellow |
|
480;565 |
578 |
yellow |
||
Rhodamine |
560 |
580 |
orange |
|
Tamara |
565 |
580 |
red |
|
Cy3.5 581 |
581 |
596 |
red |
|
Rox |
575 |
602 |
red |
|
Alexa fluor 568 |
578 |
603 |
red |
|
Red 613 |
480;565 |
613 |
red |
|
Texas Red |
615 |
615 |
red |
|
Alexa fluor 594 |
590 |
617 |
red |
|
Alexa fluor 633 |
621 |
639 |
red |
|
Allophycocyanin |
650 |
660 |
red |
|
Alexa fluor 633 |
650 |
668 |
red |
|
Cy5 |
650 |
670 |
red |
|
Alexa fluor 660 |
663 |
690 |
red |
|
Cy5.5 |
675 |
694 |
red |
|
TruRed |
490;675 |
695 |
red |
|
Alexa fluor 680 |
679 |
702 |
red |
|
Cy7 |
743 |
770 |
red |
|
Nucleic acid probes: |
|
|
|
|
Dye |
Absorbance Wavelength |
Emission Wavelength |
Visible color |
|
DAPI |
345 |
455 |
blue |
|
Hoechst 33258 |
345 |
478 |
blue |
|
SYTOX blue |
431 |
480 |
blue |
|
Hoechst 33342 |
343 |
483 |
blue |
|
509 |
509 |
green |
||
SYTOX green |
504 |
533 |
green |
|
TOTO 1, |
509 |
533 |
green |
|
SYTOX orange |
547 |
570 |
yellow |
|
Chromomycin A3 |
445 |
575 |
yellow |
|
Mithramycin |
445 |
575 |
yellow |
|
Propidium iodide |
536 |
617 |
red |
|
Ethidium bromide |
493 |
620 |
red |
|