Tools:
Bacteria Transformation Efficiency Calculator
Transformation efficiency (transformants/µg) is calculated as follows:
# colonies on plate/ng of DNA plated X 1000 ng/µg
Enter the concentration (in μg/μl) of
the DNA used to transform:
μg/μl
Enter the volume (in μl) of
DNA added to the transformation mix:
μl
Enter the volume (in μl) of
the total transformation reaction
*
:
μl
Enter the volume (in μl) actually plated
from the transformation mix (for a single plate):
μl
Enter the number of colonies or
transformants from the plate above:
Transformation Efficiency:
Transformants / μg DNA or
Transformants / ng of DNA
*
This calculation assumes that no futher dilutions of the transformation reaction are made after DNA, competent cells, and media are added.
