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Resources: Fluorochrome Absorption Emission Wavelengths

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Fluorochrome
Absorption (nm)
Emission (nm)
Other info
Haematoporphyrin
530-560
580
Hoechst 33258
345
487
Hoechst 33342
347
483
Hoechst 34580
392
440
HPTS
355
465
Hydroxycoumarin
325-360
386-455
Hydroxystilbamidine (FluoroGold)
361
536
Hydroxytryptamine
400
530
Indo-1, high calcium
330
401
Emission ratio dye
Indo-1, low calcium
346
475
Emission ratio dye
Indodicarbocyanine (DiD)
644
665
Indotricarbocyanine (DiR)
748
780
Intrawhite Cf
360
430
JC-1
514
529
JO-JO-1
530
545
JO-PRO-1
532
544
LaserPro
795
812
Laurodan
355
460
LDS 751 (DNA)
543
712
LDS 751 (RNA)
590
607
Leucophor PAF
370
430
Leucophor SF
380
465
Leucophor WS
395
465
Lissamine Rhodamine
572, 577
591, 592
Lissamine Rhodamine B
577
592
LIVE/DEAD Kit Animal Cells
Calcein/Ethidium homodimer
494
528
517
617
for more details refer
to www.probes.com
LOLO-1
566
580
LO-PRO-1
568
581
Lucifer Yellow
425, 428
528, 536, 540
Lyso Tracker Blue
373
422
Lyso Tracker Blue-White
466
536
Lyso Tracker Green
504, 534
511, 551
Lyso Tracker Red
490
516
Lyso Tracker Yellow
551
576
LysoSensor Blue
374
424
LysoSensor Green
442
505
LysoSensor Yellow/Blue
384
540
Mag Green
507
531
Magdala Red (Phloxin B)
524
600
Mag-Fura Red
483/427
659/631
Ratio Dye, Mg2+
Mag-Fura-2
369/329
369/330
508
511/491
Ratio Dye Ca2+
Ratio Dye Mg2+
Mag-Fura-5
369/330
369/332
505/500
505/482
Ratio Dye, Ca2+
Ratio Dye, Mg2+
Mag-Indo-1
349/328
349/330
480/390
480/417
Ratio Dye, Ca2+
Ratio Dye, Mg2+
Magnesium Green
506, 507
531
Magnesium Orange
550
575
Malachite Green
628
Marina Blue
362
459
Maxilon Brilliant Flavin 10 GFF
450
495
Maxilon Brilliant Flavin 8 GFF
460
495
Merocyanin
555
578
Methoxycoumarin
360
410
Mitotracker Green FM
490
516
Mitotracker Orange
551
576
Mitotracker Red
578
599
Mitramycin
450
470
Monobromobimane
398
490
Monobromobimane (mBBr-GSH)
398
500
Monochlorobimane
380
461
MPS (Methyl Green Pyronine Stilbene)
364
395
NBD
466
539
NBD Amine
450
530
Nile Red
515-555, 559
590, 640
Nitrobenzoxadidole
465
510-650
Noradrenaline
340
490-520
Nuclear Fast Red
289-530
580
Nuclear Yellow
365
495
Nylosan Brilliant Iavin E8G
460
510
Oregon Green
503
522
Oregon Green 488-X
494
517
Oregon Green™
503
522
Oregon Green™ 488
490, 493
514, 520
Oregon Green™ 500
497
517
Oregon Green™ 514
506
526
Pacific Blue
405
455
Pararosaniline (Feulgen)
570
625
PBFI
340/380
420
Excitation ratio dye
PE-Cy5
488
670
PE-Cy7
488
755, 767
PerCP
488
675
PerCP-Cy5.5
488
710
PE-TexasRed [Red 613]
488
613
Phloxin B (Magdala Red)
524
600
Phorwite AR
360
430
Phorwite BKL
370
430
Phorwite Rev
380
430
Phorwite RPA
375
430
Phosphine 3R
465
565
PhotoResist
365
610
Phycoerythrin B [PE]
546-565
575
Phycoerythrin R [PE]
565
578
PKH26 (Sigma)
551
567
PKH67
496
520
Chroma
PMIA
341
376
Pontochrome Blue Black
535-553
605
POPO-1
433
457
POPO-3
533
574
PO-PRO-1
435
455
PO-PRO-3
539
567
Primuline
410
550
Procion Yellow
470
600
Propidium Iodid (PI)
(305), 536, 538
617
PyMPO
412, 415
561, 564, 570
Pyrene
360
387
Pyronine
410
540
Pyronine B
540-590
560-650
Pyrozal Brilliant Flavin 7GF
365
495
QSY 7
560
Quinacrine Mustard
440
510
Red 613 [PE-TexasRed]
488
613
Resorufin
571
584, 585
RH 414
532
716
Rhod-2
552
576
Rhodamine
550
573
Rhodamine 110
496, 497
520
Rhodamine 123
507
529
Rhodamine 5 GLD
470
565
Rhodamine 6G
525
555
Rhodamine B
540
625
Rhodamine B 200
523-557
595
Rhodamine B extra
550
605
Rhodamine BB
540
580
Rhodamine BG
540
572
Rhodamine Green
502
527
Rhodamine Phallicidine
558, 542
575, 565
Rhodamine Phalloidine
542
565
Rhodamine Red
570
590
Rhodamine WT
530
555
Rose Bengal
525, 540
550-600
R-phycocyanine
R-phycoerythrin (PE)
565
578
rsGFP
473
509
red shifted GFP (S65T)
S65A
471
504
S65C
479
507
S65L
484
510
S65T
488
511
Sapphire GFP
395
511
SBFI
340/380
420
Excitation ratio dye
Serotonin
365
520-540
Sevron Brilliant Red 2B
520
595
Sevron Brilliant Red 4G
500
583
Sevron Brilliant Red B
530
590
Sevron Orange
440
530
Sevron Yellow L
430
490
sgBFP™
387
450
sgBFP™ (super glow BFP)
387
450
Quantum's SuperGlo™GFP
AFPs
sgGFP™
474
488
sgGFP™ (super glow GFP)
474
509
Quantum's SuperGlo™GFP
AFPs
SITS
336
436
Ion Channels
SITS (Primuline)
395-425
450
SITS (Stilbene Isothiosulphonic Acid)
365
460
SNAFL calcein
506/535
535/620
Ratio Dye, pH
SNAFL-1
508/540
543/623
Ratio Dye, pH
SNAFL-2
514/543
546/630
Ratio Dye, pH
SNARF calcein
552/574
590/629
Ratio Dye, pH
SNARF1
576/548
635/587
Excitation and emission ratio dye
Sodium Green
506, 507
532
Na+, K+
SpectrumAqua
433,/53
480/55
Vysis
SpectrumGreen
497/30, 509/31
538/44, 524/56
Vysis
SpectrumOrange
559/38, 560
588/48
Vysis
Spectrum Red
587, 587/35
612, 612/51
SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)
344
443
Stilbene
335
440
Sulphorhodamine B can C
520
595
Sulphorhodamine G Extra
470
570
SYTO 11
508, 510
527, 530
Dye for DNA, RNA
SYTO 12
499, 500
522, 519
Dye for DNA, RNA
SYTO 13
488, 491
509, 514
Dye for DNA, RNA
SYTO 14
517, 521
549, 547
Dye for DNA, RNA
SYTO 15
516, 518
546, 555
Dye for DNA, RNA
SYTO 16
488, 494
518, 525
Dye for DNA, RNA
SYTO 17
621
634
Dye for DNA
SYTO 18
490, 493
507, 527
Dye for DNA, RNA
SYTO 20
512
530
Dye for DNA
SYTO 21
494
517
Dye for DNA
SYTO 22
515
535
Dye for DNA
SYTO 23
499
520
Dye for DNA
SYTO 24
490
515
Dye for DNA
SYTO 25
521
556
Dye for DNA
SYTO 40
420
441
Dye for DNA
SYTO 41
430
454
Dye for DNA
SYTO 42
433
460
Dye for DNA
SYTO 43
436
467
Dye for DNA
SYTO 44
446
471
Dye for DNA
SYTO 45
452
484
Dye for DNA
SYTO 59
622
645
Dye for DNA
SYTO 60
652
678
Dye for DNA
SYTO 61
628
645
Dye for DNA
SYTO 62
652
676
Dye for DNA
SYTO 63
657
673
Dye for DNA
SYTO 64
599
619
Dye for DNA
SYTO 80
531
545
Nucleic Acid Stain
SYTO 81
530
544
Nucleic Acid Stain
SYTO 82
541
560
Nucleic Acid Stain
SYTO 83
543
559
Nucleic Acid Stain
SYTO 84
567
582
Nucleic Acid Stain
SYTO 85
567
583
Nucleic Acid Stain
SYTOX Blue
445
470
Nucleic Acid Stain
SYTOX Green
504
523
Nucleic Acid Stain
SYTOX Orange
547
570
Nucleic Acid Stain
Tetracycline
390-425
525-560
Tetramethylrhodamine (TRITC)
555
576
Texas Red™
595
620
Texas Red-X™ conjugate
595
615
Thiadicarbocyanine (DiSC3)
651, 653
674, 675
Thiazine Red R
596
615
Thiazole Orange
510
530
Thioflavin 5
430
550
Thioflavin S
430
550
Thioflavin TCN
350
460
Thiolyte
370-385
477-488
Thiozole Orange
453
480
Tinopol CBS (Calcofluor White)
390
430
TMR
550
573
TO-PRO-1
515
531
TO-PRO-3
644
657
TO-PRO-5
747
770
TOTO-1
514
531, 533
TOTO-3
642
660
TriColor (PE-Cy5)
(488) 650
667
TRITC TetramethylRodamineIsoThioCyanate
550
573
True Blue
365
425
TruRed
490
695
Ultralite
656
678
Uranine B
420
520
Uvitex SFC
365
435
wt GFP
395 (475)
508
wild type GFP
WW 781
605
639
X-Rhodamine
580
605
XRITC
582
601
Xylene Orange
546
580
Y66F
360
508
Y66H
360
442
Y66W
436
485
Yellow GFP
513
527
Yellow shifted
Green Fluorescent Protein
YFP
513, 520
527, 532
Yellow Fluorescent Protein
YO-PRO-1
491
506
YO-PRO-3
613
629
YOYO-1
491
508, 509
YOYO-3
612
631

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source: Carl Zeiss