AarI
AarI recognizes CACCTGC and cuts at a fixed distance downstream, leaving a 4-nucleotide overhang set by the flanking DNA.
Recognition & cut site
CACCTGC(4/8)
Properties
- Recognition site
- CACCTGC
- Cut site
- CACCTGC(4/8)
- Ends produced
- 5′ overhang
- Overhang
- variable (4 nt)
- Site length
- 7 bp
- Cuts on average
- 1 / 16,384 bp
- Palindromic
- No
- Cuts within site
- No (type IIS)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cutNone known.
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhangNone known.