Restriction Enzymes: Recognition Sites, Cut Sites and Buffers

Reference index of restriction enzymes with recognition sequences, cut sites, overhangs and buffer conditions.

219 of 219

Enzyme	Recognition site	Cut	Ends	Overhang	Cuts ~1 in
AarI	CACCTGC	CACCTGC(4/8)	5′ overhang	variable	16,384 bp
AasI	GACNNNNNNGTC	GACNNNN^NNGTC	3′ overhang	variable	4,096 bp
AatII	GACGTC	GACGT^C	3′ overhang	ACGT	4,096 bp
Acc65I	GGTACC	G^GTACC	5′ overhang	GTAC	4,096 bp
AccI	GTMKAC	GT^MKAC	5′ overhang	MK	1,024 bp
AciI	CCGC	CCGC(-3/-1)	5′ overhang	variable	256 bp
AclI	AACGTT	AA^CGTT	5′ overhang	CG	4,096 bp
AdeI	CACNNNGTG	CACNNN^GTG	3′ overhang	variable	4,096 bp
AfeI	AGCGCT	AGC^GCT	blunt	—	4,096 bp
AflII	CTTAAG	C^TTAAG	5′ overhang	TTAA	4,096 bp
AflIII	ACRYGT	A^CRYGT	5′ overhang	CRYG	1,024 bp
AgeI	ACCGGT	A^CCGGT	5′ overhang	CCGG	4,096 bp
AhdI	GACNNNNNGTC	GACNNN^NNGTC	3′ overhang	variable	4,096 bp
AleI	CACNNNNGTG	CACNN^NNGTG	blunt	—	4,096 bp
AloI	GAACNNNNNNTCC	GAACNNNNNNTCC(12/7)	3′ overhang	variable	16,384 bp
AluI	AGCT	AG^CT	blunt	—	256 bp
Alw21I	GWGCWC	GWGCW^C	3′ overhang	WGCW	1,024 bp
AlwI	GGATC	GGATC(4/5)	5′ overhang	variable	1,024 bp
AlwNI	GAGNNNCTG	CAGNNN^CTG	3′ overhang	variable	4,096 bp
ApaI	GGGCCC	GGGCC^C	3′ overhang	GGCC	4,096 bp
ApaLI	GTGCAC	G^TGCAC	5′ overhang	TGCA	4,096 bp
ApoI	RAATTY	R^AATTY	5′ overhang	AATT	1,024 bp
AscI	GGCGCGCC	GG^CGCGCC	5′ overhang	CGCG	65,536 bp
AseI	ATTAAT	AT^TAAT	5′ overhang	TA	4,096 bp
AsiSI	GCGATCGC	GCGAT^CGC	3′ overhang	AT	65,536 bp
AvaI	CYCGRG	C^YCGRG	5′ overhang	YCGR	1,024 bp
AvrII	CCTAGG	C^CTAGG	5′ overhang	CTAG	4,096 bp
BaeI	ACNNNNGTAYC	ACNNNNGTAYC(12/7)	3′ overhang	variable	8,192 bp
BamHI	GGATCC	G^GATCC	5′ overhang	GATC	4,096 bp
BanI	GGYRCC	G^GYRCC	5′ overhang	GYRC	1,024 bp
BanII	GRGCYC	GRGCY^C	3′ overhang	RGCY	1,024 bp
BbsI	GAAGAC	GAAGAC(2/6)	5′ overhang	variable	4,096 bp
BbvCI	CCTCAGC	CCTCAGC(-5/-2)	5′ overhang	variable	16,384 bp
BbvI	GCAGC	GCAGC(8/12)	5′ overhang	variable	1,024 bp
BceAI	ACGGC	ACGGC(12/14)	5′ overhang	variable	1,024 bp
BcgI	CGANNNNNNTGC	CGANNNNNNTGC(12/10)	3′ overhang	variable	4,096 bp
BclI	TGATCA	T^GATCA	5′ overhang	GATC	4,096 bp
BcnI	CCSGG	CC^SGG	5′ overhang	S	512 bp
BfaI	CTAG	C^TAG	5′ overhang	TA	256 bp
BfiI	ACTGGG	ACTGGG(5/4)	3′ overhang	variable	4,096 bp
BfmI	CTRYAG	C^TRYAG	5′ overhang	TRYA	1,024 bp
BfuAI	ACCTGC	ACCTGC(4/8)	5′ overhang	variable	4,096 bp
BfuI	GTATCC	GTATCC(6/5)	3′ overhang	variable	4,096 bp
BglI	GCCNNNNNGGC	GCCNNNN^NGGC	3′ overhang	variable	4,096 bp
BglII	AGATCT	A^GATCT	5′ overhang	GATC	4,096 bp
BlpI	GCTNAGC	GC^TNAGC	5′ overhang	variable	4,096 bp
BoxI	GACNNNNGTC	GACNN^NNGTC	blunt	—	4,096 bp
BplI	GAGNNNNNCTC	GAGNNNNNCTC(13/8)	3′ overhang	variable	4,096 bp
Bpu10I	CCTNAGC	CCTNAGC(-5/-2)	5′ overhang	variable	4,096 bp
BsaAI	YACGTR	YAC^GTR	blunt	—	1,024 bp
BsaBI	GATNNNNATC	GATNN^NNATC	blunt	—	4,096 bp
BsaHI	GRCGYC	GR^CGYC	5′ overhang	CG	1,024 bp
BsaI	GGTCTC	GGTCTC(1/5)	5′ overhang	variable	4,096 bp
BsaJI	CCNNGG	C^CNNGG	5′ overhang	variable	256 bp
BsaWI	WCCGGW	W^CCGGW	5′ overhang	CCGG	1,024 bp
BsaXI	ACNNNNNCTCC	ACNNNNNCTCC(10/7)	3′ overhang	variable	4,096 bp
BseGI	GGATG	GGATG(2/0)	3′ overhang	variable	1,024 bp
BseMI	GCAATG	GCAATG(2/0)	3′ overhang	variable	4,096 bp
BseMII	CTCAG	CTCAG(10/8)	3′ overhang	variable	1,024 bp
BseRI	GAGGAG	GAGGAG(10/8)	3′ overhang	variable	4,096 bp
BseSI	GKGCMC	GKGCM^C	3′ overhang	KGCM	1,024 bp
BseYI	CCCAGC	CCCAGC(-5/-1)	5′ overhang	variable	4,096 bp
BsgI	GTGCAG	GTGCAG(16/14)	3′ overhang	variable	4,096 bp
BsiEI	CGRYCG	CGRY^CG	3′ overhang	RY	1,024 bp
BsiWI	CGTACG	C^GTACG	5′ overhang	GTAC	4,096 bp
BslI	CCNNNNNNNGG	CCNNNNN^NNGG	3′ overhang	variable	256 bp
BsmAI	GTCTC	GTCTC(1/5)	5′ overhang	variable	1,024 bp
BsmBI	CGTCTC	CGTCTC(1/5)	5′ overhang	variable	4,096 bp
BsmFI	GGGAC	GGGAC(10/14)	5′ overhang	variable	1,024 bp
BsmI	GAATGC	GAATGC(1/-1)	3′ overhang	variable	4,096 bp
BspEI	TCCGGA	T^CCGGA	5′ overhang	CCGG	4,096 bp
BspLI	GGNNCC	GGN^NCC	blunt	—	256 bp
BsrFI	RCCGGY	R^CCGGY	5′ overhang	CCGG	1,024 bp
BsrGI	TGTACA	T^GTACA	5′ overhang	GTAC	4,096 bp
BsrI	ACTGG	ACTGG(1/-1)	3′ overhang	variable	1,024 bp
BssKI	CCNGG	^CCNGG	5′ overhang	variable	256 bp
BssSI	CACGAG	CACGAG(-5/-1)	5′ overhang	variable	4,096 bp
Bst1107I	GTATAC	GTA^TAC	blunt	—	4,096 bp
BstAPI	GCANNNNNTGC	GCANNNN^NTGC	3′ overhang	variable	4,096 bp
BstBI	TTCGAA	TT^CGAA	5′ overhang	CG	4,096 bp
BstEII	GGTNACC	G^GTNACC	5′ overhang	variable	4,096 bp
BstUI	CGCG	CG^CG	blunt	—	256 bp
BstXI	CCANNNNNNTGG	CCANNNNN^NTGG	3′ overhang	variable	4,096 bp
Bsu36I	CCTNAGG	CC^TNAGG	5′ overhang	variable	4,096 bp
BtgI	CCRYGG	C^CRYGG	5′ overhang	CRYG	1,024 bp
BtsI	GCAGTG	GCAGTG(2/0)	3′ overhang	variable	4,096 bp
Cac8I	GCNNGC	GCN^NGC	blunt	—	256 bp
CaiI	CAGNNNCTG	CAGNNN^CTG	3′ overhang	variable	4,096 bp
Cfr13I	GGNCC	G^GNCC	5′ overhang	variable	256 bp
CfrI	YGGCCR	Y^GGCCR	5′ overhang	GGCC	1,024 bp
ClaI	ATCGAT	AT^CGAT	5′ overhang	CG	4,096 bp
CpoI	CGGWCCG	CG^GWCCG	5′ overhang	GWC	8,192 bp
Csp6I	GTAC	G^TAC	5′ overhang	TA	256 bp
DdeI	CTNAG	C^TNAG	5′ overhang	variable	256 bp
DpnI	GATC	GA^TC	blunt	—	256 bp
DpnII	GATC	^GATC	5′ overhang	GATC	256 bp
DraI	TTTAAA	TTT^AAA	blunt	—	4,096 bp
EagI	CGGCCG	C^GGCCG	5′ overhang	GGCC	4,096 bp
EarI	CTCTTC	CTCTTC(1/4)	5′ overhang	variable	4,096 bp
EciI	GGCGGA	GGCGGA(11/9)	3′ overhang	variable	4,096 bp
Eco57I	CTGAAG	CTGAAG(16/14)	3′ overhang	variable	4,096 bp
Eco57MI	CTGRAG	CTGRAG(16/14)	3′ overhang	variable	2,048 bp
EcoICRI	GAGCTC	GAG^CTC	blunt	—	4,096 bp
EcoO109I	RGGNCCY	RG^GNCCY	5′ overhang	variable	1,024 bp
EcoRI	GAATTC	G^AATTC	5′ overhang	AATT	4,096 bp
EcoRV	GATATC	GAT^ATC	blunt	—	4,096 bp
EheI	GGCGCC	GGC^GCC	blunt	—	4,096 bp
FauI	CCCGC	CCCGC(4/6)	5′ overhang	variable	1,024 bp
FokI	GGATG	GGATG(9/13)	5′ overhang	variable	1,024 bp
FseI	GGCCGGCC	GGCCGG^CC	3′ overhang	CCGG	65,536 bp
FspAI	RTGCGCAY	RTGC^GCAY	blunt	—	16,384 bp
FspI	TGCGCA	TGC^GCA	blunt	—	4,096 bp
GsuI	CTGGAG	CTGGAG(16/14)	3′ overhang	variable	4,096 bp
HaeII	RGCGCY	RGCGC^Y	3′ overhang	GCGC	1,024 bp
HaeIII	GGCC	GG^CC	blunt	—	256 bp
HgaI	GACGC	GACGC(5/10)	5′ overhang	variable	1,024 bp
HhaI	GCGC	GCG^C	3′ overhang	CG	256 bp
Hin4I	GAYNNNNNVTC	GAYNNNNNVTC(13/8)	3′ overhang	variable	683 bp
Hin6I	GCGC	G^CGC	5′ overhang	CG	256 bp
HincII	GTYRAC	GTY^RAC	blunt	—	1,024 bp
HindIII	AAGCTT	A^AGCTT	5′ overhang	AGCT	4,096 bp
HinfI	GANTC	G^ANTC	5′ overhang	variable	256 bp
HpaI	GTTAAC	GTT^AAC	blunt	—	4,096 bp
HpaII	CCGG	C^CGG	5′ overhang	CG	256 bp
HphI	GGTGA	GGTGA(8/7)	3′ overhang	variable	1,024 bp
Hpy188I	TCNGA	TCN^GA	3′ overhang	variable	256 bp
Hpy188III	TCNNGA	TC^NNGA	5′ overhang	variable	256 bp
Hpy8I	GTNNAC	GTN^NAC	blunt	—	256 bp
Hpy99I	CGWCG	CGWCG^	3′ overhang	CGWCG	512 bp
HpyCH4IV	ACGT	A^CGT	5′ overhang	CG	256 bp
HpyCH4V	TGCA	TG^CA	blunt	—	256 bp
KasI	GGCGCC	G^GCGCC	5′ overhang	GCGC	4,096 bp
KpnI	GGTACC	GGTAC^C	3′ overhang	GTAC	4,096 bp
LweI	GCATC	GCATC(5/9)	5′ overhang	variable	1,024 bp
MbiI	CCGCTC	CCGCTC(-3/-3)	blunt	—	4,096 bp
MboI	GATC	^GATC	5′ overhang	GATC	256 bp
MboII	GAAGA	GAAGA(8/7)	3′ overhang	variable	1,024 bp
MfeI	CAATTG	C^AATTG	5′ overhang	AATT	4,096 bp
MluI	ACGCGT	A^CGCGT	5′ overhang	CGCG	4,096 bp
MlyI	GAGTC	GAGTC(5/5)	blunt	—	1,024 bp
MmeI	TCCRAC	TCCRAC(20/18)	3′ overhang	variable	2,048 bp
MnlI	CCTC	CCTC(7/6)	3′ overhang	variable	256 bp
MscI	TGGCCA	TGG^CCA	blunt	—	4,096 bp
MseI	TTAA	T^TAA	5′ overhang	TA	256 bp
MslI	CAYNNNNRTG	CAYNN^NNRTG	blunt	—	1,024 bp
MspA1I	CMGCKG	CMG^CKG	blunt	—	1,024 bp
MspI	CCGG	C^CGG	5′ overhang	CG	256 bp
MvaI	CCWGG	CC^WGG	5′ overhang	W	512 bp
MwoI	GCNNNNNNNGC	GCNNNNN^NNGC	3′ overhang	variable	256 bp
NaeI	GCCGGC	GCC^GGC	blunt	—	4,096 bp
NarI	GGCGCC	GG^CGCC	5′ overhang	CG	4,096 bp
NcoI	CCATGG	C^CATGG	5′ overhang	CATG	4,096 bp
NdeI	CATATG	CA^TATG	5′ overhang	TA	4,096 bp
NgoMIV	GCCGGC	G^CCGGC	5′ overhang	CCGG	4,096 bp
NheI	GCTAGC	G^CTAGC	5′ overhang	CTAG	4,096 bp
NlaIII	CATG	CATG^	3′ overhang	CATG	256 bp
NmuCI	GTSAC	^GTSAC	5′ overhang	GTSAC	512 bp
NotI	GCGGCCGC	GC^GGCCGC	5′ overhang	GGCC	65,536 bp
NruI	TCGCGA	TCG^CGA	blunt	—	4,096 bp
NsiI	ATGCAT	ATGCA^T	3′ overhang	TGCA	4,096 bp
NspI	RCATGY	RCATG^Y	3′ overhang	CATG	1,024 bp
PacI	TTAATTAA	TTAAT^TAA	3′ overhang	AT	65,536 bp
PagI	TCATGA	T^CATGA	5′ overhang	CATG	4,096 bp
PauI	GCGCGC	G^CGCGC	5′ overhang	CGCG	4,096 bp
PciI	ACATGT	A^CATGT	5′ overhang	CATG	4,096 bp
PdmI	GAANNNNTTC	GAANN^NNTTC	blunt	—	4,096 bp
PflMI	CCANNNNNTGG	CCANNNN^NTGG	3′ overhang	variable	4,096 bp
PfoI	TCCNGGA	T^CCNGGA	5′ overhang	variable	4,096 bp
PleI	GAGTC	GAGTC(4/5)	5′ overhang	variable	1,024 bp
PmeI	GTTTAAAC	GTTT^AAAC	blunt	—	65,536 bp
PmlI	CACGTG	CAC^GTG	blunt	—	4,096 bp
PpiI	GAACNNNNNCTC	GAACNNNNNCTC(13/8)	3′ overhang	variable	16,384 bp
PpuMI	RGGWCCY	RG^GWCCY	5′ overhang	GWC	2,048 bp
PsiI	TTATAA	TTA^TAA	blunt	—	4,096 bp
PspGI	CCWGG	^CCWGG	5′ overhang	CCWGG	512 bp
PspOMI	GGGCCC	G^GGCCC	5′ overhang	GGCC	4,096 bp
PstI	CTGCAG	CTGCA^G	3′ overhang	TGCA	4,096 bp
PsuI	RGATCY	R^GATCY	5′ overhang	GATC	1,024 bp
PsyI	GACNNNGTC	GACN^NNGTC	5′ overhang	variable	4,096 bp
PvuI	CGATCG	CGAT^CG	3′ overhang	AT	4,096 bp
PvuII	CAGCTG	CAG^CTG	blunt	—	4,096 bp
RsaI	GTAC	GT^AC	blunt	—	256 bp
SacI	GAGCTC	GAGCT^C	3′ overhang	AGCT	4,096 bp
SacII	CCGCGG	CCGC^GG	3′ overhang	GC	4,096 bp
SalI	GTCGAC	G^TCGAC	5′ overhang	TCGA	4,096 bp
SapI	GCTCTTC	GCTCTTC(1/4)	5′ overhang	variable	16,384 bp
SatI	GCNGC	GC^NGC	5′ overhang	variable	256 bp
Sau3AI	GATC	^GATC	5′ overhang	GATC	256 bp
SbfI	CCTGCAGG	CCTGCA^GG	3′ overhang	TGCA	65,536 bp
ScaI	AGTACT	AGT^ACT	blunt	—	4,096 bp
ScrFI	CCNGG	CC^NGG	5′ overhang	variable	256 bp
SduI	GDGCHC	GDGCH^C	3′ overhang	DGCH	455 bp
SexAI	ACCWGGT	A^CCWGGT	5′ overhang	CCWGG	8,192 bp
SfiI	GGCCNNNNNGGCC	GGCCNNNN^NGGCC	3′ overhang	variable	65,536 bp
SgrAI	CRCCGGYG	CR^CCGGYG	5′ overhang	CCGG	16,384 bp
SinI	GGWCC	G^GWCC	5′ overhang	GWC	512 bp
SmaI	CCCGGG	CCC^GGG	blunt	—	4,096 bp
SmlI	CTYRAG	C^TYRAG	5′ overhang	TYRA	1,024 bp
SnaBI	TACGTA	TAC^GTA	blunt	—	4,096 bp
SpeI	ACTAGT	A^CTAGT	5′ overhang	CTAG	4,096 bp
SphI	GCATGC	GCATG^C	3′ overhang	CATG	4,096 bp
SspI	AATATT	AAT^ATT	blunt	—	4,096 bp
StuI	AGGCCT	AGG^CCT	blunt	—	4,096 bp
StyI	CCWWGG	C^CWWGG	5′ overhang	CWWG	1,024 bp
SwaI	ATTTAAAT	ATTT^AAAT	blunt	—	65,536 bp
TaaI	ACNGT	ACN^GT	3′ overhang	variable	256 bp
TaiI	ACGT	ACGT^	3′ overhang	ACGT	256 bp
TaqI	TCGA	T^CGA	5′ overhang	CG	256 bp
TasI	AATT	^AATT	5′ overhang	AATT	256 bp
TatI	WGTACW	W^GTACW	5′ overhang	GTAC	1,024 bp
TauI	GCSGC	GCSG^C	3′ overhang	CSG	512 bp
TfiI	GAWTC	G^AWTC	5′ overhang	AWT	512 bp
TseI	GCWGC	G^CWGC	5′ overhang	CWG	512 bp
TspRI	CASTG	CASTGNN(0/-5)	3′ overhang	variable	512 bp
XagI	CCTNNNNNAGG	CCTNN^NNNAGG	5′ overhang	variable	4,096 bp
XbaI	TCTAGA	T^CTAGA	5′ overhang	CTAG	4,096 bp
XcmI	CCANNNNNNNNNTGG	CCANNNNN^NNNNTGG	3′ overhang	variable	4,096 bp
XhoI	CTCGAG	C^TCGAG	5′ overhang	TCGA	4,096 bp
XmaI	CCCGGG	C^CCGGG	5′ overhang	CCGG	4,096 bp