AfeI

AfeI cuts DNA at AGCGCT, leaving blunt ends. Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

AGC^GCT

Properties

Recognition site
AGCGCT
Cut site
AGC^GCT
Ends produced
blunt
Overhang
—
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • AitI
  • Aor51HI
  • Eco47III
  • FunI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.