AflII
AflII cuts DNA at CTTAAG, leaving a 4-nucleotide 5′ overhang (TTAA). Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition & cut site
C^TTAAG
Properties
- Recognition site
- CTTAAG
- Cut site
- C^TTAAG
- Ends produced
- 5′ overhang
- Overhang
- TTAA (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- BfrI
- BspTI
- Bst98I
- BstAFI
- BstPZ740I
- Esp4I
- MspCI
- Vha464I
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhangNone known.