AgeI

AgeI cuts DNA at ACCGGT, leaving a 4-nucleotide 5′ overhang (CCGG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

A^CCGGT

Properties

Recognition site: ACCGGT
Cut site: A^CCGGT
Ends produced: 5′ overhang
Overhang: CCGG (4 nt)
Site length: 6 bp
Cuts on average: 1 / 4,096 bp
Palindromic: Yes
Cuts within site: Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut

AsiAI
AsiGI
BshTI
CsiAI
CspAI
PinAI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang