AsiSI

AsiSI cuts DNA at GCGATCGC, leaving a 2-nucleotide 3′ overhang (AT). Its 8 bp site occurs about once every 65,536 bp of random sequence.

Recognition & cut site

GCGAT^CGC

Properties

Recognition site
GCGATCGC
Cut site
GCGAT^CGC
Ends produced
3′ overhang
Overhang
AT (2 nt)
Site length
8 bp
Cuts on average
1 / 65,536 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • RgaI
  • SfaAI
  • SgfI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • paci
  • Afa16RI
  • Afa22MI
  • BspCI
  • BspKT6I
  • BstKTI
  • EagBI
  • ErhB9I
  • MvrI
  • NblI
  • Ple19I
  • Psu161I
  • PvuI
  • RshI
  • XorII