BbsI

BbsI recognizes GAAGAC and cuts downstream of it, so the 4-nucleotide overhang is chosen by design — used in Golden Gate and MoClo assembly.

Recognition & cut site

GAAGAC(2/6)

Properties

Recognition site
GAAGAC
Cut site
GAAGAC(2/6)
Ends produced
5′ overhang
Overhang
variable (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
No
Cuts within site
No (type IIS)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • Bbv16II
  • BbvII
  • BpiI
  • BpuAI
  • Bsc91I
  • BspBS31I
  • BspIS4I
  • BspTS514I
  • BstBS32I
  • BstTS5I
  • BstV2I

Neoschizomers

same site, different cut position
  • Bbr7I

Compatible ends

different site, matching overhang

None known.