BsmI

BsmI recognizes GAATGC and cuts at a fixed distance downstream, leaving a 2-nucleotide overhang set by the flanking DNA.

Recognition & cut site

GAATGC(1/-1)

Properties

Recognition site
GAATGC
Cut site
GAATGC(1/-1)
Ends produced
3′ overhang
Overhang
variable (2 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
No
Cuts within site
No (type IIS)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • Asp26HI
  • Asp27HI
  • Asp35HI
  • Asp36HI
  • Asp40HI
  • Asp50HI
  • BsaMI
  • BscCI
  • Mva1269I
  • PctI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.