BsrGI

BsrGI cuts DNA at TGTACA, leaving a 4-nucleotide 5′ overhang (GTAC). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

T^GTACA

Properties

Recognition site
TGTACA
Cut site
T^GTACA
Ends produced
5′ overhang
Overhang
GTAC (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • AauI
  • Bsp1407I
  • BstAUI
  • Ssp4800I
  • SspBI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • bsiwi
  • Acc65I
  • AhaB8I
  • Asp718I
  • BpuB5I
  • BvuBI
  • MaeK81I
  • Pfl23II
  • PpuAI
  • PspLI
  • SplI
  • SthI
  • SunI
  • TatI