EcoRI
EcoRI cuts DNA at GAATTC, leaving a 4-nucleotide 5′ overhang (AATT). Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition & cut site
G^AATTC
Properties
- Recognition site
- GAATTC
- Cut site
- G^AATTC
- Ends produced
- 5′ overhang
- Overhang
- AATT (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- FunII
- HalI
- Kpn49kI
- Ppu111I
- RsrI
- Srl32DII
- Srl55DI
- SsoI
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhang- AcsI
- ApoI
- FsiI
- MfeI
- MluCI
- MunI
- Sse9I
- TasI
- Tsp509I
- TspEI
- XapI