GsuI

GsuI recognizes CTGGAG and cuts at a fixed distance downstream, leaving a 2-nucleotide overhang set by the flanking DNA.

Recognition & cut site

CTGGAG(16/14)

Properties

Recognition site: CTGGAG
Cut site: CTGGAG(16/14)
Ends produced: 3′ overhang
Overhang: variable (2 nt)
Site length: 6 bp
Cuts on average: 1 / 4,096 bp
Palindromic: No
Cuts within site: No (type IIS)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut

BpmI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.