HindIII

HindIII cuts DNA at AAGCTT, leaving a 4-nucleotide 5′ overhang (AGCT). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

A^AGCTT

Properties

Recognition site
AAGCTT
Cut site
A^AGCTT
Ends produced
5′ overhang
Overhang
AGCT (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • BbrI
  • BspKT8I
  • BspLAIII
  • BstFI
  • EcoVIII
  • HsuI
  • LlaCI
  • SsbI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.