MspI

MspI cuts CCGG whether or not the internal cytosine is methylated, unlike its isoschizomer HpaII. Leaves a 2-nucleotide 5′ overhang.

Recognition & cut site

C^CGG

Properties

Recognition site: CCGG
Cut site: C^CGG
Ends produced: 5′ overhang
Overhang: CG (2 nt)
Site length: 4 bp
Cuts on average: 1 / 256 bp
Palindromic: Yes
Cuts within site: Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut

hpaii
Bco27I
BsiSI
Bst40I
BsuFI
CboI
HapII
Hin2I
MnoI
Pde137I
Sth134I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang