NdeI

NdeI cuts DNA at CATATG, leaving a 2-nucleotide 5′ overhang (TA). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

CA^TATG

Properties

Recognition site
CATATG
Cut site
CA^TATG
Ends produced
5′ overhang
Overhang
TA (2 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • FauNDI
  • PfaAII

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • bfai
  • msei
  • AseI
  • AsnI
  • BpoAI
  • Csp6I
  • CviQI
  • CviRII
  • FgoI
  • FspBI
  • MaeI
  • MthZI
  • PshBI
  • RmaI
  • RsaNI
  • SaqAI
  • Sru4DI
  • SspMI
  • Tru1I
  • Tru9I
  • VspI
  • XspI