SalI

SalI cuts DNA at GTCGAC, leaving a 4-nucleotide 5′ overhang (TCGA). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

G^TCGAC

Properties

Recognition site
GTCGAC
Cut site
G^TCGAC
Ends produced
5′ overhang
Overhang
TCGA (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • BspMKI
  • HgiCIII
  • HgiDII
  • NopI
  • RflFI
  • Rtr63I
  • RtrI
  • XciI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • xhoi
  • AbrI
  • BluI
  • BspAAI
  • BssHI
  • BstVI
  • BsuMI
  • CcrI
  • MavI
  • MlaAI
  • PaeR7I
  • PanI
  • PspXI
  • Sau3239I
  • SauLPII
  • Sbi68I
  • Sfr274I
  • SlaI
  • Sol10179I
  • StrI
  • TliI
  • XpaI