SapI

SapI recognizes GCTCTTC and cuts at a fixed distance downstream, leaving a 3-nucleotide overhang set by the flanking DNA.

Recognition & cut site

GCTCTTC(1/4)

Properties

Recognition site: GCTCTTC
Cut site: GCTCTTC(1/4)
Ends produced: 5′ overhang
Overhang: variable (3 nt)
Site length: 7 bp
Cuts on average: 1 / 16,384 bp
Palindromic: No
Cuts within site: No (type IIS)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut

BspQI
LguI
PciSI
VpaK32I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang

None known.