SspI
SspI cuts DNA at AATATT, leaving blunt ends. Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition & cut site
AAT^ATT
Properties
- Recognition site
- AATATT
- Cut site
- AAT^ATT
- Ends produced
- blunt
- Overhang
- —
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cutNone known.
Neoschizomers
same site, different cut positionNone known.
Compatible ends
different site, matching overhangNone known.