AscI

AscI cuts DNA at GGCGCGCC, leaving a 4-nucleotide 5′ overhang (CGCG). Its 8 bp site occurs about once every 65,536 bp of random sequence.

Recognition & cut site

GG^CGCGCC

Properties

Recognition site
GGCGCGCC
Cut site
GG^CGCGCC
Ends produced
5′ overhang
Overhang
CGCG (4 nt)
Site length
8 bp
Cuts on average
1 / 65,536 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • PalAI
  • SgsI

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • mlui
  • Bbi24I
  • BsePI
  • BssHII
  • BstBZ153I
  • PauI
  • PteI
  • SelI