MluI

MluI cuts DNA at ACGCGT, leaving a 4-nucleotide 5′ overhang (CGCG). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

A^CGCGT

Properties

Recognition site
ACGCGT
Cut site
A^CGCGT
Ends produced
5′ overhang
Overhang
CGCG (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

Runs in your browser. Nothing is uploaded.

Related enzymes

Isoschizomers

same site, same cut
  • Bbi24I

Neoschizomers

same site, different cut position

None known.

Compatible ends

different site, matching overhang
  • asci
  • BsePI
  • BssHII
  • BstBZ153I
  • PalAI
  • PauI
  • PteI
  • SelI
  • SgsI