NsiI

NsiI cuts DNA at ATGCAT, leaving a 4-nucleotide 3′ overhang (TGCA). Its 6 bp site occurs about once every 4,096 bp of random sequence.

Recognition & cut site

ATGCA^T

Properties

Recognition site
ATGCAT
Cut site
ATGCA^T
Ends produced
3′ overhang
Overhang
TGCA (4 nt)
Site length
6 bp
Cuts on average
1 / 4,096 bp
Palindromic
Yes
Cuts within site
Yes (type II)

Sequence checker

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Related enzymes

Isoschizomers

same site, same cut
  • BfrBI
  • Csp68KIII
  • EcoT22I
  • Mph1103I
  • PinBI
  • SepI
  • SspD5II
  • Zsp2I

Neoschizomers

same site, different cut position
  • AvaIII
  • Ppu10I

Compatible ends

different site, matching overhang
  • psti
  • sbfi
  • AjoI
  • AliAJI
  • ApiI
  • Asp713I
  • BloHII
  • Bsp63I
  • BspBI
  • BspMAI
  • BsuBI
  • CflI
  • CfrA4I
  • CfuII
  • CstI
  • Ecl2zI
  • Ecl37kI
  • HalII
  • MhaAI
  • PaeHII
  • Pfl21I
  • Psp23I
  • SalPI
  • SdaI
  • SflI
  • Srl5DI
  • Sse8387I
  • Sst12I
  • YenI