SbfI
SbfI cuts DNA at CCTGCAGG, leaving a 4-nucleotide 3′ overhang (TGCA). Its 8 bp site occurs about once every 65,536 bp of random sequence.
Recognition & cut site
CCTGCA^GG
Properties
- Recognition site
- CCTGCAGG
- Cut site
- CCTGCA^GG
- Ends produced
- 3′ overhang
- Overhang
- TGCA (4 nt)
- Site length
- 8 bp
- Cuts on average
- 1 / 65,536 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
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Related enzymes
Isoschizomers
same site, same cut- SdaI
- Sse8387I
Neoschizomers
same site, different cut positionNone known.