SpeI
SpeI cuts DNA at ACTAGT, leaving a 4-nucleotide 5′ overhang (CTAG). Its 6 bp site occurs about once every 4,096 bp of random sequence.
Recognition & cut site
A^CTAGT
Properties
- Recognition site
- ACTAGT
- Cut site
- A^CTAGT
- Ends produced
- 5′ overhang
- Overhang
- CTAG (4 nt)
- Site length
- 6 bp
- Cuts on average
- 1 / 4,096 bp
- Palindromic
- Yes
- Cuts within site
- Yes (type II)
Sequence checker
Runs in your browser. Nothing is uploaded.